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Comparison of infection of different cell lines by uropathogenic Escherichia coli

  • Articles/Medical Microbiology
  • Published:
Chinese Science Bulletin

Abstract

Studying the interaction between uropathogenic Escherichia coli (UPEC) and uroepithelial cells is important in elucidating the pathogenesis of urinary tract infection. In this study, the African green monkey kidney cells (Vero), human kidney carcinoma cells (Ketr-3) and bladder carcinoma cells (EJ) were infected by UPEC132, a clinical strain isolated from Tianjin, China, and were compared for their capacities to allow the adherence and invasion by this strain. The results revealed that all these cell lines could be attached and invaded by UPEC132. The adherence rates for Vero, Ketr-3 and EJ cells were (49.20 ± 7.55)%, (55.22 ± 4.09)% and (73.20 ± 5.26)%, respectively, and invasion frequencies were (2.61 ± 0.32)×10−3, (3.00 ± 0.34)×10−3 and (3.25 ± 0.20)×10−3, respectively. The statistical analysis showed that the adherence rate for EJ cells was significantly higher than those for the other two cell lines (P<0.05), and the invasion frequencies for EJ and Ketr-3 cells had no statistical differences (P>0.05) but were higher than that for Vero cells (P<0.05). Three cell lines were detected for the receptors for P pili of UPEC by using indirect immunofluorescence. The results showed that receptors existed on the surfaces of all cell lines, and the highest distribution was found on the surface of EJ cells. Additionally, the invasion of EJ cells by recombinant UPEC132/pSELECT-GFP could be directly visualized using confocal microscopy. These data strongly implicated that EJ cells could be more easily infected by UPEC132 than the other cells, and thus could serve as a good experimental target for further investigation of UPEC infection.

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References

  1. Vaisanen V, Elo J, Tallgren L G, et al. Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet, 1981, 2: 1366–1369

    Article  Google Scholar 

  2. Wullt B, Bergsten G, Samuelsson M, et al. The role of P fimbriae for Escherichia coli establishment and mucosal inflammation in the human urinary tract. Int J Antimicrob Agents, 2002, 19: 522–538

    Article  Google Scholar 

  3. Emody L, Kerenyi M, Nagy G. Virulence factors of uropathogenic Escherichia coli. Int J Antimicrob Agents, 2003, 22(Suppl 2): 29–33

    Article  Google Scholar 

  4. Gunther N W 4th, Snyder J A, Lockatell V, et al. Assessment of virulence of uropathogenic Escherichia coli type 1 fimbrial mutants in which the invertible element is phase-locked on or off. Infect Immun, 2002, 70: 3344–3354

    Article  Google Scholar 

  5. Mysorekar I U, Hultgren S J. Mechanisms of uropathogenic Escherichia coli persistence and eradication from the urinary tract. Proc Natl Acad Sci USA, 2006, 103: 14170–14175

    Article  Google Scholar 

  6. Xie B, Zhou G, Chan S Y, et al. Distinct glycan structures of uroplakins Ia and Ib: structural basis for the selective binding of FimH adhesin to uroplakin Ia. J Biol Chem, 2006, 281: 14644–14653

    Article  Google Scholar 

  7. Brzuszkiewicz E, Brüggemann H, Liesegang H, et al. How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains. Proc Natl Acad Sci USA, 2006, 103: 12879–12884

    Article  Google Scholar 

  8. Poljakovic M, Svensson L, Persson K. The influence of uropathogenic Escherichia coli and proinflammatory cytokines on the inducible nitric oxide synthase response in human kidney epithelial cells. J Urol, 2005, 173: 1000–1003

    Article  Google Scholar 

  9. Schilling J D, Martin S M, Hunstad D A, et al. CD14- and Toll-like receptor-dependent activation of bladder epithelial cells by lipopolysaccharide and type 1 piliated Escherichia coli. Infect Immun, 2003, 71: 1470–1480

    Article  Google Scholar 

  10. Hunstad D A, Justice S S, Hung C S, et al. Suppression of bladder epithelial cytokine responses by uropathogenic Escherichia coli. Infect Immun, 2005, 73: 3999–4006

    Article  Google Scholar 

  11. Falzano L, Filippini P, Travaglione S, et al. Escherichia coli cytotoxic necrotizing factor 1 blocks cell cycle G2/M transition in uroepithelial cells. Infect Immun, 2006, 74: 3765–3772

    Article  Google Scholar 

  12. Zheng L, Chen J Y, Chen Y K, et al. Cloning and sequencing of adhesin gene papG derived from uropathogenic Escherichia coli (in Chinese). Chinese J Zoonoses, 2002, 18: 34–38

    Google Scholar 

  13. Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989

    Google Scholar 

  14. Birosova E, Siegfried L, Kmet’ova M, et al. Detection of virulence factors in alpha-haemolytic Escherichia coli strains isolated from various clinical materials. Clin Microbiol Infect, 2004, 10: 569–573

    Article  Google Scholar 

  15. Bahrani-Mougeot F K, Buckles E L, Lockatell C V, et al. Type 1 fimbriae and extracellular polysaccharides are preeminent uropathogenic Escherichia coli virulence determinants in the murine urinary tract. Mol Microbiol, 2002, 45: 1079–1093

    Article  Google Scholar 

  16. Das M, Hart-Van Tassell A, Urvil P T, et al. Hydrophilic domain II of Escherichia coli Dr fimbriae facilitates cell invasion. Infect Immun, 2005, 73: 6119–6126

    Article  Google Scholar 

  17. Marcus D M, Kundu, S K, Suzuki A. The P blood group system: Recent progress in immunochemistry and genetics. Semin Hematol, 1981, 18: 63–71

    Google Scholar 

  18. O’Toole C M, Povey S, Hepburn P, et al. Identity of some human bladder cancer cell lines. Nature, 1983, 301: 429–430

    Article  Google Scholar 

  19. Snyder J A, Lloyd A L, Lockatell C V, et al. Role of phase variation of type 1 fimbriae in a uropathogenic Escherichia coli cystitis isolate during urinary tract infection. Infect Immun, 2006, 74: 1387–1393

    Article  Google Scholar 

  20. Holden N J, Totsika M, Mahler E, et al. Demonstration of regulatory cross-talk between P fimbriae and type 1 fimbriae in uropathogenic Escherichia coli. Microbiology, 2006, 152: 1143–1153

    Article  Google Scholar 

  21. Martinez J J, Mulvey M A, Schilling J D, et al. Type 1 pilus-mediated bacterial invasion of bladder epithelial cells. EMBO J, 2000, 19: 2803–2812

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Medical Microbiology, Tianjin Medical University, Tianjin, 300070, China

    Xin Ge, Jie Dong, JinYing Chen, Ping Yao, Chao Gu & DongJing Yang

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  1. Xin Ge
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  2. Jie Dong
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  3. JinYing Chen
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  4. Ping Yao
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  5. Chao Gu
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  6. DongJing Yang
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Corresponding author

Correspondence to JinYing Chen.

Additional information

Supported by the National Natural Science Foundation of China (Grant No. 30470096) and Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20070062010)

Contributed equally to this work

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Ge, X., Dong, J., Chen, J. et al. Comparison of infection of different cell lines by uropathogenic Escherichia coli . Chin. Sci. Bull. 54, 2662–2668 (2009). https://doi.org/10.1007/s11434-009-0389-7

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  • DOI: https://doi.org/10.1007/s11434-009-0389-7

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