Skip to main content
SpringerLink
Log in

A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity

  • Articles/Molecular Virology
  • Published:
Chinese Science Bulletin

Abstract

The complete genome of Marek’s disease virus (MDV) strain GX0101, which was integrated with the LTR sequences of REV, was cloned in Escherichia coli as a bacterial artificial chromosome (BAC). BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination. The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B. Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast (CEF). This BAC viral clone was named bac-GX0101. When the reconstituted virus was inoculated into 1-day-old birds, visceral tumors could be detected as early as 62 d post infection. There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus. The BAC derived virus maintained its oncogenicity and immunosuppressive effects. In conclusion, the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued. With the powerful BAC manipulation system, the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Isfort R, Jones D, Kost R, et al. Retrovirus insertion into herpesvirus in vitro and in vivo. Proc Ntal Acad Sci USA, 1992, 89: 991–995

    Article  Google Scholar 

  2. Davidson I, Borenshtain R. In vivo events of retroviral long terminal repeat integration into Marek’s disease virus in commercial poultry: Detection of chimeric molecules as a marker. Avian Dis, 2001, 45: 102–121

    Article  Google Scholar 

  3. Zhang Z, Cui Z. Isolation of recombinant field strains of Marek’s disease virus integration with reticuloendotheliosis virus genome fragments. Sci China C-Life Sci, 2005, 48: 81–88

    Article  Google Scholar 

  4. Witter R L, Silver R F, Lee L F. New serotype 2 and attenuated serotype 1 Marek’s disease vaccine viruses: Selected biological and molecular characteristics. Avian Dis, 1987, 31: 829–840

    Article  Google Scholar 

  5. Schumacher D, Tischer B K, Fuchs W, et al. Reconstitution of Marek’s disease virus serotype 1(MDV-1) from DNA cloned as a bacterial artificial chromosome and characterization of a glycoprotein B-negative MDV-1 mutant. J Virol, 2000, 74: 11088–11098

    Article  Google Scholar 

  6. Petherbridge L, Homes K, Baigent S J, et al. Replication-competent bacterial artificial chromosomes of Marek’s disease virus: Novel tools for generation of molecularly defined herpesvirus vaccines. J Virol, 2003, 77: 8712–8718

    Article  Google Scholar 

  7. Petherbridge L, Brown A C, Baigent S J, et al. Oncogenicity of virulent Marek’s disease virus cloned as bacterial artificial chromosomes. J Virol, 2004, 78: 13376–13380

    Article  Google Scholar 

  8. Baigent S J, Petherbridge L, Smith L P, et al. Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces protection against Marek’s disease. J Gen Virol, 2006, 87: 769–776

    Article  Google Scholar 

  9. Zhao Y G, Petherbridge L, Smith L P, et al. Self-excision of the BAC cequence from the recombinant Marek’s disease virus genome increases replication and pathogenicity. Virol J, 2008, 5: 19

    Article  Google Scholar 

  10. Cui H Y, Wang Y F, Shi X M et al. Construction of Marek’s disease virus serotype 814 strain as an infectioous bacterial artificial chromosome. Chin J Biotech, 2008, 24: 569–575

    Article  Google Scholar 

  11. Morgan R W, Cantello J L, McDermott C H, et al. Transfection of chicken embryo fibroblasts with Marek’s disease virus DNA. Avian Dis, 1990, 34: 345–351

    Article  Google Scholar 

  12. Muyrers J P, Zhang Y, Stewart A F, et al. Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Res, 1999, 27: 1555–1557

    Article  Google Scholar 

  13. Narayaman K, Williamson R, Zhang Y, et al. Efficiant and engineering of a 200 kb beta-globin human bacterial chromsome in E. coli DH10B using an inducible homologous recombination system. Gene Ther, 1999, 6: 442–447

    Google Scholar 

  14. Sambrook J, Fritsch D F, Maniatis T, et al. Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989

    Google Scholar 

  15. Lee L F, Liu X, Witter R L. Monoclonal antibodies with specially for three different serotypes of Marek’s disease viruses in chickens. J Immunol, 1983, 130: 1003–1006

    Google Scholar 

  16. Sun S H, Cui Z Z, Sun A J, et al. Comparisons of Preventive Effects of Maternal Antibody on Immunosuppression Induced by Homologous and Heterologous Reticuloendotheliosis Viruses. Acta Veterinaria Et Zootechnica Sinica, 2007, 38: 488–492

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Animal Science & Technology, Shandong Agricultural University, Taian, 271018, China

    AiJun Sun & ZhiZhong Cui

  2. Institute for Animal Health, Compton, RG20 7NN, UK

    Petherbridge Lawrence, YuGuang Zhao & Venugopal K. Nair

  3. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    YanPeng Li

Authors
  1. AiJun Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Petherbridge Lawrence
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. YuGuang Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. YanPeng Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Venugopal K. Nair
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. ZhiZhong Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to ZhiZhong Cui.

Additional information

Supported by the National Natural Science Foundation of China (Grant No. 30671571) and BBSRC China Partnering Award (Grant No. CPA1740)

About this article

Cite this article

Sun, A., Lawrence, P., Zhao, Y. et al. A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity. Chin. Sci. Bull. 54, 2641–2647 (2009). https://doi.org/10.1007/s11434-009-0364-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-009-0364-3

Keywords

Search

Navigation