Skip to main content
SpringerLink
Log in

Distribution of bone marrow stem cells in large porous polyester scaffolds

  • Special Topic/Articles/Biomedical Materials
  • Published:
Chinese Science Bulletin

Abstract

This work examined the optimal syringing depth during in vitro cell loading in order to even cell distribution after syringing a drop of cell suspension in cylinder poly(lactide-co-glycolide) (PLGA) porous scaffolds. The scaffolds of 10 mm height and 10 mm diameter were fabricated via room-temperature compression molding & particulate leaching technique based on spherical porogens. In vitro tests were employed for such examinations: a global observation of a cell-loaded scaffold stained by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique and a quantitative measurement of spatial distribution of cells after slicing the cell-loaded scaffolds into layers. It was found that an even distribution of cells was soon achieved only if the initial cell suspension was seeded on the layer that was below the top surface but above the middle of scaffolds. The availability of in vitro osteoblastic differentiation of rat bone marrow stem cells in such a kind of spherical-pore PLGA scaffolds was meanwhile confirmed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Crane G M, Ishaug S L Mikos A G. Bone tissue engineering. Nat Med, 1995, 1(12): 1322–1324

    Article  Google Scholar 

  2. Langer R, Vacanti J P. Tissue engineering. Science, 1993, 260(5110): 920–926

    Article  Google Scholar 

  3. Hutmacher D W. Scaffolds in tissue engineering bone and cartilage. Biomaterials, 2000, 21: 2529–2543

    Article  Google Scholar 

  4. Nair L S, Laurencin C T. Biodegradable polymers as biomaterials. Prog Polym Sci, 2007, 32: 762–798

    Article  Google Scholar 

  5. Zhang J Y, Doll B A, Beckman E J, et al. Three-dimensional biocompatible ascorbic acid-containing scaffold for bone tissue engineering. Tissue Eng 2003, 9: 1143–1157

    Article  Google Scholar 

  6. Jiao Y P, Cui F Z. Surface modification of polyester biomaterials for tissue engineering. Biomed Mater, 2007, 2: R24–R37

    Article  Google Scholar 

  7. Zhu H G, Ji J, Barbosa M A, et al. Protein electrostatic self-assembly on poly(DL-Lactide) scaffold to promote osteoblas growth. J Biomed Mater Res Part B, 2004, 71B: 159–165

    Article  Google Scholar 

  8. Yang Z J, Yuan H P, Tong W D, et al. Osteogenesis in extraskeletally implanted porous calcium phosphate ceramics: Variability among different kinds of animals. Biomaterials, 1996, 17: 2131–2137

    Article  Google Scholar 

  9. Cancedda R, Giannoni P, Mastrogiacomo M. A tissue engineering approach to bone repair in large animal models and in clinical practice. Biomaterials, 2007, 28: 4240–4250

    Article  Google Scholar 

  10. Ma Z W, Gao C Y, Gong Y H, et al. Paraffin spheres as porogen to fabricate poly(L-lactic acid) scaffolds with improved cytocompatibility for cartilage tissue engineering. J Biomed Mater Res Part B, 2003, 67B: 610–617

    Article  Google Scholar 

  11. Wang M, Chen W, Zhang H, et al. Synthesis and characterization of PLLA-PLCA-PEG multiblock copolymers and their applications in modifying PLLA porous scaffolds. Euro Polym J, 2007, 43: 4683–4694

    Article  Google Scholar 

  12. Ishaug S L, Crane G M, Miller M J, et al. Bone formation by threedimensional stromal osteoblast culture in biodegradable polymer scaffolds. J Biomed Mater Res, 1997, 36: 17–28

    Article  Google Scholar 

  13. Wu L B, Zhang H, Zhang J C, et al. Fabrication of three-dimensional porous scaffolds with complicated shape for tissue engineering. I. compression molding based on flexible-rigid combined mould. Tissue Eng, 2005, 11: 1105–1114

    Google Scholar 

  14. Wu L B, Jing D Y, Ding J D. A “room-temperature” injection molding/particulate leaching approach for fabrication of biodegradable threedimensional porous scaffolds. Biomaterials, 2006, 27: 185–191

    Article  Google Scholar 

  15. Wu L B, Zhang J C, Jing D Y, et al. “Wet-state” mechanical properties of three-dimensional polyester porous scaffolds. J. Biomed Mater Res Part A, 2006, 76A: 264–271

    Article  Google Scholar 

  16. Wu L B, Ding J D. In vitro degradation of three-dimensional porous poly (D, L-lactide-co-glycolide) scaffolds for tissue engineering. Biomaterials, 2004, 25: 5821–5830

    Article  Google Scholar 

  17. Chen J W, Wang C Y, Lu S H, et al. In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells. Cell Tissue Res, 2005, 319: 429–438

    Article  Google Scholar 

  18. Leung L, Chan C, Baek S, et al. Comparison of morphology and mechanical properties of PLGA bioscaffolds. Biomed. Mater, 2008, 3: 25006

    Article  Google Scholar 

  19. Yang Y F, Zhao J, Zhao Y H, et al. Formation of porous PLGA scaffolds by a combining method of thermally induced phase separation and porogen leaching. J Appl Polym Sci, 2008, 109: 1232–1241

    Article  Google Scholar 

  20. Hu X X, Shen H, Yang F, et al. Preparation and cell affinity of microtubular orientation-structured PLGA(70/30) blood vessel scaffold. Biomaterials, 2008, 29: 3128–3136

    Article  Google Scholar 

  21. Zhang J C, Zhang H, Wu L B, et al. Fabrication of three dimensional polymeric scaffolds with spherical pores. J Mater Sci, 2006, 41: 1725–1731

    Article  Google Scholar 

  22. Zhang J C, Wu L B, Jing D Y, et al. A comparative study of porous scaffolds with cubic and spherical macropores. Polymer, 2005, 46: 4979–4985

    Google Scholar 

  23. Ma P X, Choi J W. Biodegradable polymer scaffolds with well-defined interconnected spherical pore network. Tissue Eng, 2001, 7: 23–33

    Article  Google Scholar 

  24. Chen V J, Ma P X. Nano-fibrous poly (L-lactic acid) scaffolds with interconnected spherical macropores. Biomaterials, 2004, 25: 2065–2073

    Article  Google Scholar 

  25. Holy C E, Shoichet M S, Davies J E. Engineering three-dimensional bone tissue in vitro using biodegradable scaffolds: Investigating initial cell-seeding density and culture period. J Biomed Mater Res, 2000, 51: 376–382

    Article  Google Scholar 

  26. Kim B S, Putnam A J, Kulik T J, et al. Optimizing seeding and culture methods to engineer smooth muscle tissue on biodegradable polymer matrices. Biotechnol Bioeng, 1998, 57: 46–54

    Article  Google Scholar 

  27. Reilly G C, Radin S, Chen A T, et al. Differential alkaline phosphatase responses of rat and human bone marrow derived mesenchymal stem cells to 45S5 bloactive glass. Biomaterials, 2007, 28: 4091–4097

    Article  Google Scholar 

  28. Ciapetti G, Ambrosio L, Marletta G, et al. Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering. Biomaterials, 2006, 27: 6150–6160

    Article  Google Scholar 

  29. Wang Z, Peng R, Ding J D. Periodically discontinuous induction of bone marrow stem cells toward osteogenic differentiation in vitro. Biotechnol Prog, 2008, 24: 766–772

    Article  Google Scholar 

  30. Liu G P, Zhao L, Cui L, et al. Tissue-engineered bone formation using human bone marrow stromal cells and novel ss-tricalcium phosphate. Biomed Mater, 2007, 2: 78–86

    Article  Google Scholar 

  31. Goldstein A S, Juarez T M, Helmke C D, et al. Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds. Biomaterials, 2001, 22: 1279–1288

    Article  Google Scholar 

  32. Huang W B, Carlsen B, Wulur I, et al. BMP-2 exerts differential effects on differentiation of rabbit bone marrow stromal cells grown in two-dimensional and three-dimensional systems and is required for in vitro bone formation in a PLGA scaffold. Exp Cell Res, 2004, 299: 325–334

    Article  Google Scholar 

  33. Aubin J E. Bone stem cells. J Cell Biochem, 1998, Supplements 30/31(73–82)

  34. Galban C J, Locke B R. Effects of spatial variation of cells and nutrient and product concentrations coupled with product inhibition on cell growth in a polymer scaffold. Biotechnol Bioeng, 1999, 64: 633–643

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Department of Macromolecular Science, Fudan University, Shanghai, 200433, China

    Zhen Wang, Zheng Zhang, JunChuan Zhang & JianDong Ding

  2. Department of Anatomy, Embryology and Histology, Shanghai Medical School, Fudan University, Shanghai, 200032, China

    ZhenJue She

Authors
  1. Zhen Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zheng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. JunChuan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. ZhenJue She
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. JianDong Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to JianDong Ding.

Additional information

Supported by the State Key Development Program of Basic Research of China (Grant No.2009CB930000) and National Natural Science Foundation of China (Grant No. 20774000)

About this article

Cite this article

Wang, Z., Zhang, Z., Zhang, J. et al. Distribution of bone marrow stem cells in large porous polyester scaffolds. Chin. Sci. Bull. 54, 2968–2975 (2009). https://doi.org/10.1007/s11434-009-0181-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-009-0181-8

Keywords

Search

Navigation