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Isolation and functional analysis of transcription factor GmWRKY57B from soybean

  • Articles/Molecular Biology
  • Published:
Chinese Science Bulletin

Abstract

WRKY proteins are a large family of transcriptional regulators in higher plants involved in many biological processes, such as responses to biotic and abiotic stress, plant development and metabolism. In this study, a soybean cDNA library was constructed and screened by yeast one-hybrid system using the 3×W-box element as a bait, and consequently a transcription factor, named GmWRKY57B, a member of group 3 of WRKY family, was obtained. GmWRKY57B specifically binds to the W-box of the Arabidopsis NPR1 (AtNPR1) promoter in yeast cells. This specific binding was further confirmed with an in vitro electrophoretic mobility shift assay. RT-PCR analysis showed that GmWRKY57B was expressed at a similar level in root, stem and leaf of the soybean plant. Overexpression of the GmWRKY57B gene in tobacco conferred the transgenics tolerance to drought stress. This result indicated that the GmWRKY57B gene plays a role in plant tolerance to abiotic stress.

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Authors and Affiliations

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, 100081, China

    Lan Zhang, ChunYi Zhang, YunLiu Fan & Lei Wang

  2. Department of Biology, Harbin Normal University, Harbin, 150025, China

    XiaoPing Wang & YingDong Bi

Authors
  1. Lan Zhang
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  2. XiaoPing Wang
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  3. YingDong Bi
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  4. ChunYi Zhang
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  5. YunLiu Fan
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  6. Lei Wang
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Corresponding author

Correspondence to Lei Wang.

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Contributed equally to this work

Supported by the National Basic Research and Development Program of China (Grant No. 2004CB117200) and the Basic Research Programme of Education of Heilongjiang Province (Grant No. 10541088)

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Zhang, L., Wang, X., Bi, Y. et al. Isolation and functional analysis of transcription factor GmWRKY57B from soybean. Chin. Sci. Bull. 53, 3538–3545 (2008). https://doi.org/10.1007/s11434-008-0483-2

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  • DOI: https://doi.org/10.1007/s11434-008-0483-2

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