Chinese Science Bulletin

, Volume 53, Issue 13, pp 1988–1995 | Cite as

Cloning and sequence analysis of Sox genes in a tetraploid cyprinid fish, Tor douronensis

  • BaoCheng Guo
  • JunBing Li
  • ChaoBo Tong
  • ShunPing HeEmail author
Articles Animal Genetics


A PCR survey for Sox genes in a young tetraploid fish Tor douronensis (Teleostei: Cyprinidae) was performed to access the evolutionary fates of important functional genes after genome duplication caused by polyploidization event. Totally 13 Sox genes were obtained in Tor douronensis, which represent SoxB, SoxC and SoxE groups. Phylogenetic analysis of Sox genes in Tor douronensis provided evidence for fish-specific genome duplication, and suggested that Sox19 might be a teleost specific Sox gene member. Sequence analysis revealed most of the nucleotide substitutions between duplicated copies of Sox genes caused by tetraploidization event or their orthologues in other species are silent substitutions. It would appear that the sequences are under purifying selective pressure, strongly suggesting that they represent functional genes and supporting selection against all null allele at either of two duplicated loci of Sox4a, Sox9a and Sox9b. Surprising variations of the intron length and similarities of two duplicated copies of Sox9a and Sox9b, suggest that Tor douronensis might be an allotetraploidy.


Tor douronensis Sox gene tetraploidy genome duplication gene diversity 


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Copyright information

© Science in China Press and Springer-Verlag GmbH 2008

Authors and Affiliations

  • BaoCheng Guo
    • 1
    • 2
  • JunBing Li
    • 1
    • 2
  • ChaoBo Tong
    • 1
    • 2
  • ShunPing He
    • 1
    Email author
  1. 1.Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  2. 2.Graduate University of the Chinese Academy of SciencesBeijingChina

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