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DNA methylation status of H19 and Xist genes in lungs of somatic cell nuclear transfer bovines

  • Articles
  • Animal Genetics
  • Published:
Chinese Science Bulletin

Abstract

In somatic cell nuclear transfer (SCNT) technologies, the donor cell’s nuclei need to be epigenetically reprogrammed for embryonic development. The incomplete reprogramming of donor cell nuclei has been implicated as a primary reason for the low efficiency of SCNT. DNA methylation is a major epigenetic modification of the genome that regulates crucial aspects of genome function, including establishment of genomic imprinting. In order to make sure whether the DNA methylation reprogramming is efficient in SCNT animals, we analyzed the DNA methylation status of two imprinting genes, H19 and Xist, in lungs of deceased SCNT bovines that died within 48 h of birth using bisulfite sequencing analysis. Our findings demonstrated that cloned bovines showed significantly lower DNA methylation of H19 than controls (P<0.05), and three tested CpGs sites (1, 2, 3) exhibited unmethylation in one cloned bovine (9C3); however, Xist showed similar DNA methylation levels between clones and controls, and both showed hypermethylation (96.11% and 86.67%).

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Authors and Affiliations

  1. College of Life Science, Hebei Agriculture University, Baoding, 071001, China

    Jie Chen, YanQin Liu, Cui Zhang & ShiJie Li

  2. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100094, China

    YunPing Dai, ShiJie Li & Ning Li

  3. College of Life Science and Life Engineering, Hebei Science and Technology University, Shijiazhuang, 050018, China

    DongJie Li

Authors
  1. Jie Chen
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  2. DongJie Li
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  3. YanQin Liu
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  4. Cui Zhang
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  5. YunPing Dai
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  6. ShiJie Li
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  7. Ning Li
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Corresponding authors

Correspondence to ShiJie Li or Ning Li.

Additional information

Supported by the National High Technology Research and Development Program of China (Grant No. 2001AA213091) and Natural Science Foundation of Hebei Province (Grant No. C2006001032)

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Chen, J., Li, D., Liu, Y. et al. DNA methylation status of H19 and Xist genes in lungs of somatic cell nuclear transfer bovines. Chin. Sci. Bull. 53, 1996–2001 (2008). https://doi.org/10.1007/s11434-008-0249-x

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  • DOI: https://doi.org/10.1007/s11434-008-0249-x

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