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Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

  • Articles
  • Molecular Virology
  • Published:
Chinese Science Bulletin

Abstract

An Buthus martensii Karsch Insect Toxin (BmK IT) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 × 107 PlBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6 h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8 h) during a 192 h infection.

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Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, China

    XiaoJun Fan, Bo Zheng, YueJun Fu, Yi Sun & AiHua Liang

Authors
  1. XiaoJun Fan
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  2. Bo Zheng
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  3. YueJun Fu
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  4. Yi Sun
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  5. AiHua Liang
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Corresponding author

Correspondence to AiHua Liang.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 30670282 and 30700534) and the Department of Science and Technology of Shanxi Province

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Fan, X., Zheng, B., Fu, Y. et al. Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy. Chin. Sci. Bull. 53, 1855–1860 (2008). https://doi.org/10.1007/s11434-008-0242-4

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  • DOI: https://doi.org/10.1007/s11434-008-0242-4

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