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Chinese Science Bulletin

, Volume 53, Issue 11, pp 1671–1674 | Cite as

Application of a TLC chemical method to detection of cyclotides in plants

  • Xu WenYan
  • Tang Jun
  • Ji ChangJiu
  • He WenJun
  • Tan NingHua
Articles Chemical Biology

Abstract

Iodine, Dragendorff’s reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography (TLC). Cyclotides could be distinguished from the others by comparing the TLC coloring plots with G-250 and ninhydrin reagents. With this method, cyclotides were discovered in Viola labridorica, V. tricolor, V. hamiltoniana, Momordica charantia, and M. cochinchinensis and over 10 cyclotides were isolated, three of which were determined as known cyclotides cycloviolacin O2, kalata B1 and vary peptide A.

Keywords

cyclotides TLC chemical detection method coomassie brilliant blue G-250 

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Copyright information

© Science in China Press and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Xu WenYan
    • 1
    • 2
  • Tang Jun
    • 1
    • 2
  • Ji ChangJiu
    • 1
    • 2
  • He WenJun
    • 1
    • 2
  • Tan NingHua
    • 1
  1. 1.State Key Laboratory of Phytochemistry and Plant Resources in West ChinaKunming Institute of Botany, Chinese Academy of SciencesKunmingChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina

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