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β2-Adrenoceptor affinity chromatography and its application in the screening of the active compounds from Semen Armeniacae Amarum

  • Articles
  • Analytical Chemistry
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Chinese Science Bulletin

Abstract

β2-Adrenoceptor (β2-AR) was purified from the rabbit lung tissue by sepharose-salbutamol affinity chromatographic column. To prepare the β2-AR stationary phase, β2-AR was evenly immobilized on the surface of macro-pore silica with a mild chemical coupling method through covalent bond. The retention properties of β2-AR stationary phase were characterized by four ligands, salbutamol sulfate, noradrenaline bitartrate, adrenaline hydrochloride and propranolol hydrochloride, to establish the β2-AR affinity chromatography. Then, the method was used to screen the active compounds from the total extracts of Semen Armeniacae Amarum. The results showed that β2-AR on the surface of the stationary phase could keep its original bioactivity and selectivity. Amygdalin retained in the chromatographic column was proved to be the active compound of the total extracts of Semen Armeniacae Amarum. Compared with the existing chromatographic screening approaches, this method showed a good stability and high selectivity. The active compounds which could interact with β2-AR in traditional Chinese medicine (TCM) could be screened efficiently by this method, providing a new way to screen the active compounds in complicated samples such as TCM.

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Authors and Affiliations

  1. Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Institute of Analytical Science, Northwest University, Xi’an, 710069, China

    XiaoHui Zheng, Rong Yang, ShiXiang Wang, YinMao Wei & JianBin Zheng

  2. Engineering and Research Center of Chinese Herb Modernization, Xi’an Jiaotong University, Xi’an, 710061, China

    XinFeng Zhao

Authors
  1. XiaoHui Zheng
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  2. XinFeng Zhao
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  3. Rong Yang
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  4. ShiXiang Wang
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  5. YinMao Wei
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  6. JianBin Zheng
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Corresponding author

Correspondence to JianBin Zheng.

Additional information

Supported by the National Natural Science Foundation of China (Grant No. 20575052) and the Natural Science Foundation of Shaanxi Province, China (Grant Nos. 2006B03 and 2006C220)

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Zheng, X., Zhao, X., Yang, R. et al. β2-Adrenoceptor affinity chromatography and its application in the screening of the active compounds from Semen Armeniacae Amarum . Chin. Sci. Bull. 53, 842–847 (2008). https://doi.org/10.1007/s11434-007-0510-8

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  • DOI: https://doi.org/10.1007/s11434-007-0510-8

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