Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR

Abstract

Nanoparticle PCR is a novel method to optimize DNA amplification. It performs well in improving specificity, enhancing sensitivity and speed. Several mechanisms were proposed in previous studies: one was based on the interaction between gold nanoparticles (AuNPs) and DNA while the other was attributed to the heat transfer property of AuNPs. In this paper, we propose that the interaction between AuNPs and DNA polymerase can significantly influence PCR. First, the addition of DNA polymerase can eliminate the inhibitory effects of excess AuNPs. Second, the addition of AuNPs will increase yield of the desired PCR product and make the optimum concentration of DNA polymerase move to higher value. Third, while excess polymerase might inhibit amplification efficiency, AuNPs can reverse this process and the yield of PCR amplification. Based on these results we propose a possible mechanism that AuNPs might modulate the activity of polymerase and improve PCR amplification.

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Correspondence to Hu Jun or Fan ChunHai.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 10335070, 60537030, and 20404016), the Major State Research Development Pro-gram of China (Grant No. 2006CB933000), Shanghai Municipal Commission for Science and Technology (Grant No. 0652NM006) and the Chinese Academy of Sciences

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Mi, L., Zhu, H., Zhang, X. et al. Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR. CHINESE SCI BULL 52, 2345–2349 (2007). https://doi.org/10.1007/s11434-007-0327-5

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Keywords

  • PCR
  • nanoparticles
  • Au/gold nanoparticle
  • polymerase
  • mechanism)