Chinese Science Bulletin

, Volume 52, Issue 17, pp 2345–2349 | Cite as

Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR

Articles Chemical Biology

Abstract

Nanoparticle PCR is a novel method to optimize DNA amplification. It performs well in improving specificity, enhancing sensitivity and speed. Several mechanisms were proposed in previous studies: one was based on the interaction between gold nanoparticles (AuNPs) and DNA while the other was attributed to the heat transfer property of AuNPs. In this paper, we propose that the interaction between AuNPs and DNA polymerase can significantly influence PCR. First, the addition of DNA polymerase can eliminate the inhibitory effects of excess AuNPs. Second, the addition of AuNPs will increase yield of the desired PCR product and make the optimum concentration of DNA polymerase move to higher value. Third, while excess polymerase might inhibit amplification efficiency, AuNPs can reverse this process and the yield of PCR amplification. Based on these results we propose a possible mechanism that AuNPs might modulate the activity of polymerase and improve PCR amplification.

Keywords

PCR nanoparticles Au/gold nanoparticle polymerase mechanism) 

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Copyright information

© Science in China Press 2007

Authors and Affiliations

  1. 1.Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghaiChina
  2. 2.Bio-X Life Research Center, College of Life Science and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina
  3. 3.Graduate University of Chinese Academy of SciencesBeijingChina

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