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Cloning of genes encoding nonhost hypersensitive response-inducing elicitors from Phytophthora boehmeriae

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Chinese Science Bulletin

Abstract

We have devised a high-throughput functional cloning method to isolate cDNAs from Phytophthora boehmeriae of which the products elicit a hypersensitive response (HR) in tobacco. The cDNAs were cloned into a binary potato virus X (PVX)-based expression vector and transformed into Agrobacterium tumefeciens (Mog101). 4100 colonies were individually toothpick-inoculated onto leaflets of Nicotiana benthamiana. 12 cDNAs were identified whose expression induced formation of a necrotic lesion around the inoculation site. 7 of these clones have different sequences. One of these clones PBC43 encodes specific elicitin. Clone PBC163 encodes a protein highly homologous to Rab; PBC241 encodes a prohibitin protein; PBN62 encodes a Heat Shock Protein 60 (HSP60). The other five cDNAs reveal no homology to known protein and are thus considered novel. These observations suggest that this functional screening method is a versatile strategy to identify cDNAs of pathogens that encode elicitors and other HR-inducing proteins.

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Authors and Affiliations

  1. Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China

    Li Jun, Zhang HaiFeng, Zhang ZhengGuang, Wang YuanChao & Zheng XiaoBo

  2. Suzhou Plant Protection and Quarantine Station, Suzhou, 215006, China

    Li Jun

Authors
  1. Li Jun
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  2. Zhang HaiFeng
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  3. Zhang ZhengGuang
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  4. Wang YuanChao
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  5. Zheng XiaoBo
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Correspondence to Zhang ZhengGuang or Zheng XiaoBo.

Additional information

Supported by the Nation Natural Science Foundation of China (Grant No. 30300228), the National Research Foundation for the Doctoral Program of Higher Education of China (Grant No. 20020307035), and the Student Research Training of Nanjing Agricultural University (Grant No. 0402A04)

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Li, J., Zhang, H., Zhang, Z. et al. Cloning of genes encoding nonhost hypersensitive response-inducing elicitors from Phytophthora boehmeriae . CHINESE SCI BULL 52, 231–237 (2007). https://doi.org/10.1007/s11434-007-0030-6

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  • DOI: https://doi.org/10.1007/s11434-007-0030-6

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