Abstract
Using the immuno-fluorescence and immuno-gold electron microscope technology, localization of nitric oxide synthase (NOS)-like proteins was determined in guard cells of Vicia faba L. NOS is mainly localized in nucleus, cytoplasm, chloroplast, mitochondria and the cell wall of guard cells. Scorch and exogenous JA can enhance the level of nitric oxide (NO) and increase NOS activity in both leaf and epidermis, and the changing pattern of NOS activity was consistent with the change of NO. NOS inhibitor, L-NAME, inhibited JA-induced NO generation. From the results, we presumed that NO generation from NOS pathway is the main pathway in the stress and JA responses. The pharmacological experiment showed that increasing the Ca2+ at a suitable concentration promoted leaf NOS activity and the NO level, indicating that NOS activity together with the distribution of NO is Ca2+-dependent. NOS and NO are possibly involved in the regulation of stomatal movement thus playing an important role in plant stress responses.
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References
Crawford N M, Guo F Q. New insights into nitric oxide metabolism and regulatory function. Trends Plant Sci, 2005, 10(4): 195–200
Crawford N M. Mechanisms for nitric oxide synthesis in plants. J Exp Bot, 2006, 57(3): 471–478
Yamasaki H, Sakihama Y, Takahhashi S. An alternative pathway for nitric oxide production in plants: New features of an old enzyme. Trends Plant Sci, 1999, 4(4): 128–129
Guo F Q, Okamoto M, Crawford N M, et al. Identification of a plant nitric oxide synthase gene involved in hormonal signaling. Science, 2003, 302(5642): 100–103
Barroso J B, Corpas F J, Carteras A, et al. Localization of nitric-oxide synthase in plant peroxisomes. J Biol Chem, 1999, 274(51): 36729–36733
Ninneman H, Maier J. Indications for the occurrence of nitric oxide synthases in fungi and plants and the involvement in phtotconidiation of Neurospora crassa. Phtotchem Photobiol, 1996, 64: 393–398
Pedroso M C, Magalhaes J R, Durzan D. Nitric oxide induces cell death in Taxus cells. Plant Sci, 2000, 157(2): 173–180
Corpas F J, Barroso J B, Carreras A, et al. Cellular and subcellular localization of endogenous nitric oxide in young and senescent pea plants. Plant Physil, 2004, 136: 2722–2733
Delledonne M, Xia Y, Dixon R A, et al. Nitric oxide functions as a signal in plant disease resistance. Nature, 1998, 394: 585–588
Durner J, Wendehenne D, Klessig D F. Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose. Proc Natl Acad Sci USA, 1998, 95(17): 10328–10333
Huang J S, Knopp J A. Involvement of nitric oxide in Ralstonia solanacearum-induced hypersensitive reaction on tobacco. In: Prior P, Elphinstone J, Allen C, eds. Bacterial Wilt Disease: Molecular and Ecological Aspects. Berlin: INRA and Springer Edition, 1998. 218–224
Wojtaszek P. Nitric oxide in plants: To NO or not to NO. Phytochemistry, 2000, 54(1): 1–4
Huang X., Stettmaier K, Michel C, et al. Nitric oxide is induced by wounding and influences jasmonic acid signaling in Arabidopsis thaliana. Planta, 2004, 218: 938–946
Liu X, Shi W, Zhang S, et al. Nitric oxide involved in signal transduction of jasmonic acid-induced stomatal closure of Vicia faba L. Chin Sci Bull, 2005, 50(6): 520–525
Neill S J, Desikan R, Clarke A, et al. Nitric oxide is a novel component of abscisic acid signaling in stomatal guard cells. Plant Physiol, 2002, 128(1): 13–16
Foissner I, Wendehenne D, Langebartels C, et al. In vivo imaging of an elicitor-induced nitric oxide burst in tobacco. Plant J, 2000, 23: 817–824
Ribeiro Jr E A, Cunha F Q, Tamashiro W M S C, et al. Growth phase-dependent subcellular localization of nitric oxide synthase in maize cells. FEBS Lett, 1999, 445: 283–286
Guo F Q, Crawford N M. Arabidopsis nitric oxide synthase1 is targeted to mitochondria and protects against oxidative damage and dark-induced senescence. Plant Cell, 2005, 17(12): 3436–3450
Garces H, Durzan D, Petroso M C. Mechanical stress elicits nitric oxide formation and DNA fragmentation in Arabidopsis thaliana. Ann Bot, 2001, 87(5): 567–574
Cetuo M, Hernandez-Perera O, Martin R, et al. Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus. FEBS Lett, 1996, 398(2–3): 159–164
Modolo L V, Cunha F Q, Braga M R, et al. Nitric oxide synthase-mediated phytoalexin accumulation in soybean cotyledons in response to the Diaporthe phaseolorum f. sp. meridionalis elicitor. Plant Physiol, 2002, 130(3): 1288–1297
Corpas F J, Barroso J B, Carreras A, et al. Constitutive arginine-dependent nitric oxide synthase activity in different organs of pea seedlings during plant development. Planta, 2006, 6: 1–9
He Y, Tang R H, Stevens R D, et al. Nitric oxide represses the floral transition. Science, 2004, 305: 1968–1971
Desikan R, Reynolds A, Hancock J T, et al. Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on defense gene expression in Arabiodopsis suspension cultures. Biochem J, 1998, 330: 115–120
Tun N N, Santa-Catarina C, Begum T S et al. Polyamines Induce Rapid Biosynthesis of Nitric Oxide (NO) in Arabidopsis thaliana Seedlings. Plant Cell Physiol, 2006, 47(3): 346–354
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Supported by the National Natural Science Foundation of China (Grant No. 30370141)
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Liu, X., Wang, Y., Jia, W. et al. Localization of NOS-like protein in guard cells of Vicia faba L. and its possible function. CHINESE SCI BULL 52, 84–90 (2007). https://doi.org/10.1007/s11434-007-0024-4
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DOI: https://doi.org/10.1007/s11434-007-0024-4