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Transgene directionally integrated into C-genome of Brassica napus

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Chinese Science Bulletin

Abstract

Integration of a transgene into a C-genome chromosome plays an important role in reducing ecological risk of transgenic Brassica napus. To obtain C-genome transgenic B. napus, herbicide-resistant bar gene was firstly transferred into B. oleracea var. alboglabra mediated by Agrobacterium tumefaciens strain LBA4404. Then using the transgenic B. oleracea as paternal plants and 8 non-transgenic varieties of B. rapa as maternal plants, C-genome transgenic B. napus with bar gene was artificially resynthesized by means of ovary culture and chromosome doubling. Among 67 lines of the resynthesized B. napus, 31 were positive, and 36 were negative according to PCR test for bar gene. At least 2 plants from each line were kept for PPT spray confirmation. The result was in consistence with the PCR test. Genomic Southern blotting of three randomly chosen lines also showed that bar gene had been integrated into the genome of resynthesized B. napus lines.

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Authors and Affiliations

  1. Key Laboratory of Oil Crops Genetic Improvement of the Ministry of Agriculture, Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China

    Li Jun, Fang Xiaoping, Wang Zhuan, Li Jun, Luo Lixia & Hu Qiong

Authors
  1. Li Jun
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  2. Fang Xiaoping
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  3. Wang Zhuan
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  4. Li Jun
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  5. Luo Lixia
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  6. Hu Qiong
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Correspondence to Fang Xiaoping.

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Li, J., Fang, X., Wang, Z. et al. Transgene directionally integrated into C-genome of Brassica napus . CHINESE SCI BULL 51, 1578–1585 (2006). https://doi.org/10.1007/s11434-006-2021-4

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  • DOI: https://doi.org/10.1007/s11434-006-2021-4

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