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Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format

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Abstract

In this work, we parallelly detected the specific binding between microarray targets including 12 different kinds of proteins and the probe solution containing five corresponding antibodies and quantitatively analyzed the interactions between CDH13 and solution phase anti-CDH13 at six different probe concentrations by oblique-incidence reflectivity difference (OIRD) method in label-free format. The detection sensitivity reached 10 ng/mL. The experimental results indicate that the OIRD method is a promising and competing technique not only in research work but also in clinic.

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Authors and Affiliations

  1. Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China

    Jun Dai, LiPing He, HuiBin Lu, KuiJuan Jin & GuoZhen Yang

  2. Collaborative Innovation Center of Quantum Matter, Beijing, 100190, China

    KuiJuan Jin & GuoZhen Yang

  3. Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Lin Li & KangCheng Ruan

Authors
  1. Jun Dai
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  2. Lin Li
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  3. LiPing He
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  4. KangCheng Ruan
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  5. HuiBin Lu
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  6. KuiJuan Jin
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  7. GuoZhen Yang
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Correspondence to HuiBin Lu.

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Dai, J., Li, L., He, L. et al. Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format. Sci. China Phys. Mech. Astron. 57, 2039–2042 (2014). https://doi.org/10.1007/s11433-014-5481-6

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  • DOI: https://doi.org/10.1007/s11433-014-5481-6

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