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Label-free high-throughput and real-time detections of protein interactions by oblique-incidence reflectivity difference method

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Abstract

Selected Mouse IgG of 1 mg/mL as target was fabricated on microarray for 500 sample dots. Label-free and real-time reaction dynamic processes were detected between the microarrays with Goat Anti-mouse IgG of 0.02 mg/mL using the oblique-incidence reflectivity difference (OIRD) method. We obtained the reaction results and the reaction dynamic curves of 500 protein dots. In addition, we also used label-free detection of protein microarrays of 10080 sample dots, including BSA and different concentrations of Mouse IgG and Rabbit IgG, by OIRD. The obtained reaction results between the protein microarray with 1 mg/mL Goat Anti-mouse IgG and 1 mg/mL Goat Anti-rabbit IgG are reported herein. Experimental results show that OIRD can be not only label-free high-throughput detection method for biological microarrays but also label-free real-time detection in the interaction processes of biomolecules.

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References

  1. Zhu H, Bilgin M, Bangham R, et al. Global analysis of protein activities using proteome chips. Science, 2001, 293: 2101–2105

    Article  ADS  Google Scholar 

  2. Espina V, Woodhouse E C, Wulfkuhle J, et al. Protein microarray detection strategies: Focus on direct detection technologies. J Immunol Methods, 2004, 290(1): 121–133

    Article  Google Scholar 

  3. Yu X B, Xu D K, Cheng Q. Label-free detection methods for protein microarrays. Proteomics, 2006, 6(20): 5493–5503

    Article  Google Scholar 

  4. Ray S, Mehta G, Srivastava S. Label-free detection techniques for protein microarrays: Prospects, merits and challenges. Proteomics, 2010, 10(4): 731–748

    Article  Google Scholar 

  5. Han M, Gao X, Su J Z, et al. Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules. Nat Biotechnol, 2001, 19(7): 631–635

    Article  Google Scholar 

  6. Gao X, Cui Y, Levenson R M. In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol, 2004, 22(8): 969–976

    Article  Google Scholar 

  7. Li P Y, Lin B, Gerstenmaier J, et al. A new method for label-free imaging of biomolecular interactions. Sens Actuators B, 2004, 99(1): 6–13

    Article  Google Scholar 

  8. Lu H, Wen J, Wang X, et al. Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method. J Opt, 2010, 12(9): 095301

    Article  ADS  Google Scholar 

  9. Lu H, Wen J, Wang X, et al. Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method. Sci China-Phys Mech Astron, 2010, 53(7): 1230–1233

    Article  ADS  Google Scholar 

  10. Wang X, Yuan K, Lu H, et al. Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method. J Appl Phys, 2010, 107(6): 063109

    Article  ADS  Google Scholar 

  11. Yuan K, Wang X, Lu H, et al. Label-free detection of hybridization of oligonucleotides by oblique-incidence reflectivity difference method. Sci China-Phys Mech Astron, 2010, 53(7): 1434–1437

    Article  ADS  MATH  Google Scholar 

  12. Wang J Y, Dai J, He L P, et al. Label-free and real-time detections of the interactions of swine IgG with goat anti-swine IgG by oblique-incidence reflectivity difference technique. J Appl Phys, 2012, 112(6): 064702

    Article  ADS  Google Scholar 

  13. Sun Y, He L P, Dai J, et al. Label-free and real-time detection of specific binding of IgG proteins by oblique-incidence reflectivity difference method. Chin Sci Bull, 2012, 57(22): 2898–2900

    Article  Google Scholar 

  14. He L P, Dai J, Sun Y, et al. Label-free and real-time detection of antigen-antibody capture processes using the oblique-incidence reflectivity difference technique. Chin Phys Lett, 2012, 29(7): 070702

    Article  ADS  Google Scholar 

  15. He L P, Sun Y, Dai J, et al. Label-free and real-time detection of interactional dynamic processes of rabbit IgG with different concentrations and goat anti-rabbit IgG by oblique-incidence reflectivity difference method. Acta Phys Sin, 2012, 61: 060703

    Google Scholar 

  16. He L P, Sun Y, Dai J, et al. Label-free and real-time detection of antigen-antibody interactions by oblique-incidence reflectivity difference (OIRD) method. Sci China-Phys Mech Astron, 2012, 55(9): 1585–1588

    Article  ADS  Google Scholar 

  17. Dai J, Li L, Wang J Y, et al. Label-free detection repeatability of protein microarrays by oblique-incidence reflectivity difference method. Sci China-Phys Mech Astron, 2012, 55(12): 2347–2350

    Article  ADS  Google Scholar 

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Authors and Affiliations

  1. Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China

    LiPing He, Shuang Liu, Jun Dai, HuiBin Lü, KuiJuan Jin & GuoZhen Yang

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  1. LiPing He
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  2. Shuang Liu
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  3. Jun Dai
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  4. HuiBin Lü
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  5. KuiJuan Jin
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  6. GuoZhen Yang
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Correspondence to HuiBin Lü.

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He, L., Liu, S., Dai, J. et al. Label-free high-throughput and real-time detections of protein interactions by oblique-incidence reflectivity difference method. Sci. China Phys. Mech. Astron. 57, 615–618 (2014). https://doi.org/10.1007/s11433-013-5384-y

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  • DOI: https://doi.org/10.1007/s11433-013-5384-y

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