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Regulation of shear stress on rolling behaviors of HL-60 cells on P-selectin

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Abstract

Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1), and regulated by hemodynamics. Poor data were reported in understanding P-selectin-mediated rolling. With the flow chamber technique, we herein observed HL-60 cell rolling on P-selectin with or without 3% Ficoll at various wall shear stresses from 0.05 to 0.4 dyn/cm2. The results demonstrated that force rather than transport regulated the rolling, similar to rolling on L- and E-selectin. The rolling was accelerated quickly by an increasing force below the optimal shear threshold of 0.15 dyn/cm2 first and then followed by a slowly decelerating phase starting at the optimum, showing a catch-slip transition and serving as a mechanism for the rolling. The catch-slip transition was completely reflected to the tether lifetime and other rolling parameters, such as the mean and fractional stop time. The narrow catch bond regime stabilized the rolling quickly, through steeply increasing fractional stop time to a plateau of about 0.85. Data presented here suggest that the low shear stress threshold serves as a mechanism for most cell rolling events through P-selectin.

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Authors and Affiliations

  1. School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China

    YingChen Ling

  2. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China

    YingChen Ling, Ying Fang, XiaoFang Yang, QuHuan Li, QinYong Lin & JianHua Wu

  3. Institute of Biomechanics, South China University of Technology, Guangzhou, 510006, China

    Ying Fang, QuHuan Li, QinYong Lin & JianHua Wu

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  1. YingChen Ling
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Correspondence to JianHua Wu.

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Ling, Y., Fang, Y., Yang, X. et al. Regulation of shear stress on rolling behaviors of HL-60 cells on P-selectin. Sci. China Phys. Mech. Astron. 57, 1998–2006 (2014). https://doi.org/10.1007/s11433-013-5270-7

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  • DOI: https://doi.org/10.1007/s11433-013-5270-7

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