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A robust polyacrylic acid/chitosan cryogel for rapid hemostasis

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Abstract

Currently, it is a challenge to develop hemostatic materials with high water absorption capacity and anti-fatigue properties for quickly preventing massive hemorrhage from arteries and visceral organs. A series of polyacrylic acid/chitosan (PAA/CS) cryogels were prepared by a cryostructurization technique to improve mechanical performance and hemostatic efficiency of chitosan (CS). In this system, the chemically cross-linked PAA network was used as a framework to improve water absorption behaviors and mechanical strength. The CS network was co-blended by hydrogen bonding and electrostatic interactions, both of which synergistically promoted hemostasis. These cryogels had high porosity (>94%), rapid water absorption rate (<3 s), high blood absorption capacity (>2000%), outstanding mechanical strength, and fatigue resistance. Moreover, the results of cytotoxicity and hemolysis demonstrated that the cryogels had good biocompatibility. Notably, the PAA/CS cryogels exhibited superior whole blood coagulation ability and red blood cell and platelet adhesion ability compared to those of commercial hemostatic dressing (gauze, gelatin sponges, and CS sponges). Based on these results, mouse femoral artery hemorrhage models and liver hemorrhage models were prepared to investigate the hemostatic ability of the prepared PAA/CS cryogels. Results suggested that the hemostatic ability of PAA5/CS cryogels was superior to that of commercial hemostatic materials. Therefore, the PAA/CS cryogels showed potential application in preventing massive hemorrhage from arteries and visceral organs.

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Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China

    MingYue Shi, ChaoJie Yu, XiaoRu Dong, QingYu Yu, MengMeng Yao, ShaoShuai He, FangLian Yao, Hong Zhang & JunJie Li

  2. School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, 063210, China

    LiJie Jiang, ZhiWei Yue & Hong Sun

  3. Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, 300350, China

    FangLian Yao, Hong Zhang & JunJie Li

Authors
  1. MingYue Shi
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  2. LiJie Jiang
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  3. ChaoJie Yu
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  4. XiaoRu Dong
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  5. QingYu Yu
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  10. Hong Zhang
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  11. Hong Sun
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  12. JunJie Li
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Corresponding authors

Correspondence to Hong Zhang, Hong Sun or JunJie Li.

Additional information

This work was supported by the National Natural Science Foundation of China (Grant Nos. U20A20261, 31870948, 31971250, and 52073205) and Natural Science Foundation of Tianjin (Grant No. 20JCYBJC00660).

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The supporting information is available online at https://tech.scichina.com and https://link.springer.com. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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Shi, M., Jiang, L., Yu, C. et al. A robust polyacrylic acid/chitosan cryogel for rapid hemostasis. Sci. China Technol. Sci. 65, 1029–1042 (2022). https://doi.org/10.1007/s11431-021-1986-9

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  • DOI: https://doi.org/10.1007/s11431-021-1986-9

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