Skip to main content
Log in

Living concrete with self-healing function on cracks attributed to inclusion of microorganisms: Theory, technology and engineering applications—A review

  • Review
  • Published:
Science China Technological Sciences Aims and scope Submit manuscript

Abstract

Concrete is the most widely used composite material in civil engineering. Microbial induced calcium carbonate precipitation (MICP) is a green and environmental friendly technology, which has received extensive attention in repair of concrete cracks. This paper introduces the research progress in Southeast University research in past 16 years. In the early stage, MICP technology of urea hydrolyzed by Bacillus pasteurii was mainly investigated to repair the surface cracks and to fill large-size cracks with grouting. However, aiming at the hidden cracks that were difficult for human intervention, a new mineralization route of Bacillus mucilaginosus was proposed, which could repair faster than Bacillus alcalophilus, and the problem of ammonia emission in the repair process of Bacillus pasteurii was also solved. In addition, in order to improve the protection of bacteria and the self-healing efficiency of the later age cracks, the methods of fiber immobilization, carrier uniformly immobilization and core-shell structural immobilization had been compared and studied. The results showed that core-shell structural immobilization had good protection ability and strong designability. What’s more, the paper also summarized the characteristics of spore germination, cell activity, nucleation and biological calcium carbonate in crack zone, and introduced the application experience of microbial self-healing concrete in water conservancy projects and subway stations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Cheng X S, Ma L, Yu D P, et al. Seismic stability of loess tunnels under the effects of rain seepage and a train load. Sci China Tech Sci, 2018, 61: 735–747

    Article  Google Scholar 

  2. Qian C X, Wang Y J, Wang H, et al. Research on water seepage flow in concrete (in Chinese). J Build Mater, 2009, 12: 515–518

    Google Scholar 

  3. Marsavina L, Audenaert K, De Schutter G, et al. Experimental and numerical determination of the chloride penetration in cracked concrete. Constr Build Mater, 2009, 23: 264–274

    Article  Google Scholar 

  4. Kwon S J, Na U J, Park S S, et al. Service life prediction of concrete wharves with early-aged crack: Probabilistic approach for chloride diffusion. Struct Saf, 2009, 31: 75–83

    Article  Google Scholar 

  5. He R, Zheng S, Gan V J L, et al. Damage mechanism and interfacial transition zone characteristics of concrete under sulfate erosion and dry-wet cycles. Constr Build Mater, 2020, 255: 119340

    Article  Google Scholar 

  6. Hu B, Zhang N L, Liao Y T, et al. Enhanced flexural performance of epoxy polymer concrete with short natural fibers. Sci China Tech Sci, 2018, 61: 1107–1113

    Article  Google Scholar 

  7. Carević V, Ignjatović I. Influence of loading cracks on the carbonation resistance of RC elements. Constr Build Mater, 2019, 227: 116583

    Article  Google Scholar 

  8. Huang X, Tang S B, Tang C A. Boundary-effect and scale-heating rate equivalence effect of cracking behavior in the rock models subjected to heating from the central borehole. Sci China Tech Sci, 2020, 63: 809–818

    Article  Google Scholar 

  9. Liu Y, Liu X, Li G, et al. Herbivory effects on leaf litter decomposition vary with specific leaf area in temperate mixed deciduous forest. Land Degrad Dev, 2021, 32: 757–765

    Article  Google Scholar 

  10. Ferris F G, Stehmeier L G, Kantzas A, et al. Bacteriogenic mineral plugging. J Can Pet Technol, 1996, 35: 56–61

    Article  Google Scholar 

  11. Kantzas A, Stehmeier L G, Marentette D F, et al. A novel method of sand consolidation through bacteriogenic mineral plugging. In: Proceedings of CIM Annual Technical Conference. Calgary, 1992

  12. Ramachandran S K, Ramakrishnan V, Bang S S. Remediation of concrete using micro-organisms. ACI Mater J, 2001, 98: 3–9

    Google Scholar 

  13. Wang R X, Qian C X, Wang J Y. Study on microbiological precipitation of CaCO3 (in Chinese). J Southeast Univ, 2005, 35: 191–195

    Google Scholar 

  14. Qian C X, Wang R X, Wang J Y. Preparation of calcium carbonate by microbial deposition (in Chinese). China Patent, CN200510094744.5. 2006-05-30

  15. Qian C X, Wang J Y, Wang R X, et al. Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii. Mater Sci Eng-C, 2009, 29: 1273–1280

    Article  MathSciNet  Google Scholar 

  16. Wang J Y, Qian C X, Wang R X, et al. Application of carbonate-mineralization bacterium in surface protection of cement based materials (in Chinese). J Chin Ceramic Soci, 2009, 37: 1097–1102

    Google Scholar 

  17. Wang R, Qian C. In situ Restoration of the surface defects on cement-based materials by bacteria mineralization with spraying method. J Wuhan Univ Technol-Mat Sci Edit, 2014, 29: 518–526

    Article  Google Scholar 

  18. Wang R X, Qian C X. Restoration of defects on cement-based materials surface by bacteria immobilized in agar (in Chinese). J Build Mater, 2013, 16: 942–948

    MathSciNet  Google Scholar 

  19. Wang R X, Qian C X, Wang J Y, et al. Different treated methods of microbiologically deposited CaCO3 layer on hardened cement paste surface (in Chinese). J Chin Ceramic Soci, 2008, 36: 1378–1384

    Google Scholar 

  20. Qian C, Wang R, Cheng L, et al. Theory of microbial carbonate precipitation and its application in restoration of cement-based materials defects. Chin J Chem, 2010, 28: 847–857

    Article  Google Scholar 

  21. Wang R X, Qian C X. Restoration of defects on the surface of cement-based materials by microbiologically precipitated CaCO3 (in Chinese). J Chin Ceramic Soc, 2008, 36: 37–44

    Google Scholar 

  22. Cheng L, Qian C X, Wang R X, et al. Study on the mechanism of calcium carbonate formation induced by carbonate-mineralization microbe (in Chinese). Acta Chim Sin, 2007, 65: 2133–2138

    Google Scholar 

  23. Qian C, Ren L, Xue B, et al. Bio-mineralization on cement-based materials consuming CO2 from atmosphere. Constr Build Mater, 2016, 106: 126–132

    Article  Google Scholar 

  24. Qian C X, Ren L F, Luo M. Development of concrete surface defects and cracks repair technology based on microbial-induced mineralization (in Chinese). J Chin Ceramic Soc, 2015, 43: 619–631

    Google Scholar 

  25. Zhang J, Zhang Z, Hu Y Q, et al. Research progress of wound healing (in Chinese). Chin J Orthop, 2005, 25: 58–60

    Google Scholar 

  26. Azzimonti B C, Sabbatini M, Rimondini L, et al. Manipulating the healing response. Wound Heal Biomater, 2016, 1: 101–116

    Article  Google Scholar 

  27. Yang H. Morphological study of nomal gekko spinal cord and regeneration after injury (in Chinese). Dissertation of Doctoral Degree. Nantong: Nantong University, 2006

    Google Scholar 

  28. Novianti T, Juniantito V, Jusuf A A, et al. High expressions of the cytoglobin and PGC-1α genes during the tissue regeneration of house gecko (Hemidactylus platyurus) tails. BMC Dev Biol, 2020, 20: 1–26

    Article  Google Scholar 

  29. Picker A, Nicoleau L, Burghard Z, et al. Mesocrystalline calcium silicate hydrate: A bioinspired route toward elastic concrete materials. Sci Adv, 2017, 3: e1701216

    Article  Google Scholar 

  30. Speck O, Schlechtendahl M, Borm F, et al. Humidity-dependent wound sealing in succulent leaves of Delosperma cooperi—An adaptation to seasonal drought stress. Beilstein J Nanotechnol, 2018, 9: 175–186

    Article  Google Scholar 

  31. Mazur E, Benková E, Friml J. Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. Sci Rep, 2016, 6: 33754

    Article  Google Scholar 

  32. Sena G, Wang X, Liu H Y, et al. Organ regeneration does not require a functional stem cell niche in plants. Nature, 2009, 457: 1150–1153

    Article  Google Scholar 

  33. Jonkers H M, Schlangen E. Crack repair by concrete-immobilized bacteria. In: Proceedings of the First International Conference on Self-Healing Materials. Amsterdam, 2007. 18–20

  34. Qian C X, Pan Q F. Additive for crack self-repairing cement-base material, use method thereof and cement-base material (in Chinese). China Patent, CN201210052914.3. 2012-03-02

  35. Qian C X, Luo M, Pan Q F, et al. Mechanism of microbially induced calcite precipitation in self-healing concrete (in Chinese). J Chin Ceramic Soc, 2013, 42: 620–626

    Google Scholar 

  36. Qian C X, Li R Y, Pan Q F, et al. Microbial self-healing effects of concrete cracks (in Chinese). J Southeast Univ, 2013, 43: 360–364

    Google Scholar 

  37. Qian C, Li R, Luo M, et al. Distribution of calcium carbonate in the process of concrete self-healing. J Wuhan Univ Technol-Mat Sci Edit, 2016, 31: 557–562

    Article  Google Scholar 

  38. Luo M, Qian C, Li R. Factors affecting crack repairing capacity of bacteria-based self-healing concrete. Constr Build Mater, 2015, 87: 1–7

    Article  Google Scholar 

  39. Zhang Y, Qian C X, Zhang X. Physicochemical characteristics of crack mouth solution in microbial self-healing mortar (in Chinese). J Southeast Univ, 2020, 50: 101–108

    Google Scholar 

  40. Qian C X, Zhang X. New Microbial Cement (in Chinese). Beijing: Science Press, 2019. 32–35

    Google Scholar 

  41. Qian C X, Ren L F, Rong H, et al. Method for repairing crack of cement-based material (in Chinese). China Patent, CN201310301782.8. 2013-07-18

  42. Qian C, Chen H, Ren L, et al. Self-healing of early age cracks in cement-based materials by mineralization of carbonic anhydrase microorganism. Front Microbiol, 2015, 6: 1225

    Article  Google Scholar 

  43. Chen H C, Qian C X, Ren L F. Self-healing of early age cracks in cement-based materials based on mineralization of microorganism (in Chinese). J Southeast Univ, 2016, 46: 606–611

    Google Scholar 

  44. Ling H, Qian C. Effects of self-healing cracks in bacterial concrete on the transmission of chloride during electromigration. Constr Build Mater, 2017, 144: 406–411

    Article  Google Scholar 

  45. Su Y, Feng J, Zhan Q, et al. Non-ureolytic microbial self-repairing concrete for low temperature environment. Smart Mater Struct, 2019, 28: 075041

    Article  Google Scholar 

  46. Qian C X, Cao T J. Cement-based material whiskering resistance enhancement method (in Chinese). China Patent, CN201410610689.X. 2014-11-03

  47. Xue B, Qian C. Mitigation of efflorescence of wallboard by means of bio-mineralization. Front Microbiol, 2015, 6: 1155

    Article  Google Scholar 

  48. Qian C, Wang J, Cao T, et al. The mathematical model and numerical calculation method of efflorescence. Struct Concrete, 2018, 19: 1428–1435

    Article  Google Scholar 

  49. Cao T J, Qian C X, Guo J Q, et al. Research on quantitative characterization and restrain method of efflorescence for cement-based external wall system (in Chinese). China Concrete Cement Prod, 2016: 72–77

  50. Feng J, Su Y, Qian C. Coupled effect of PP fiber, PVA fiber and bacteria on self-healing efficiency of early-age cracks in concrete. Constr Build Mater, 2019, 228: 116810

    Article  Google Scholar 

  51. Su Y, Feng J, Jin P, et al. Influence of bacterial self-healing agent on early age performance of cement-based materials. Constr Build Mater, 2019, 218: 224–234

    Article  Google Scholar 

  52. Qian C X, Feng J H, Su Y L. Microbially induced calcium carbonate precipitation improves the early-age mechanical performance and self-healing effect of cement-based materials (in Chinese). Mater Rep, 2019, 33: 1983–1988

    Google Scholar 

  53. Luo M, Qian C. Influences of bacteria-based self-healing agents on cementitious materials hydration kinetics and compressive strength. Constr Build Mater, 2016, 121: 659–663

    Article  Google Scholar 

  54. Luo M, Qian C X. Performance of two bacteria-based additives used for self-healing concrete. J Mater Civ Eng, 2016, 28: 04016151

    Article  Google Scholar 

  55. Luo M. Preoperties and microstructure of self-healing cement-based materials by microbial mineralization (in Chinese). Dissertation of Doctoral Degree. Nanjing: Southeast University, 2017

    Google Scholar 

  56. Ren L F. Research on restoration of early age cracks in cement-based materials by microbe (in Chinese). Dissertation of Masteral Degree. Nanjing: Southeast University, 2015

    Google Scholar 

  57. Kanth B K, Min K, Kumari S, et al. Expression and characterization of codon-optimized carbonic anhydrase from dunaliella species for CO2 sequestration application. Appl Biochem Biotechnol, 2012, 167: 2341–2356

    Article  Google Scholar 

  58. Liu L, Li F C, Li L, et al. Carbonate minerals promoted by bacterial carbonic anhydrase (in Chinese). Carsologica Sin, 2017, 6: 433–440

    Google Scholar 

  59. Xiao L, Lian B. Heterologously expressed carbonic anhydrase from Bacillus mucilaginosus promoting CaCO3 formation by capturing atmospheric CO2. Carbonates Evaporites, 2016, 31: 39–45

    Article  Google Scholar 

  60. Su Y, Qian C, Zheng T, et al. A prepared easily bio-carrier based on chitosan modified polypropylene fibers. Biochem Eng J, 2021, 165: 107824

    Article  Google Scholar 

  61. Su Y, Qian C, Rui Y, et al. Exploring the coupled mechanism of fibers and bacteria on self-healing concrete from bacterial extracellular polymeric substances (EPS). Cement Concrete Compos, 2021, 116: 103896

    Article  Google Scholar 

  62. Zheng T, Su Y, Qian C, et al. Low alkali sulpho-aluminate cement encapsulated microbial spores for self-healing cement-based materials. Biochem Eng J, 2020, 163: 107756

    Article  Google Scholar 

  63. Zhang X, Qian C. A new type capsule-based healing agent for concrete and its protective function of spores. Smart Mater Struct, 2020, 29: 105035

    Article  Google Scholar 

  64. Qian C X, Chen H C. A preparation method of cement-based materials with deep self-healing function (in Chinese). China Patent, ZL201410470387.7. 2016-03-16

  65. Yi H H. Effect and mechanism of microorganism on volume stability and utilization efficiency of steel slag cementitious materials (in Chiese). Dissertation of Doctoral Degree. Nanjing: Southeast University, 2020

    Google Scholar 

  66. Su Y, Zheng T, Qian C. Application potential of Bacillus megaterium encapsulated by low alkaline sulphoaluminate cement in self-healing concrete. Constr Build Mater, 2021, 273: 121740

    Article  Google Scholar 

  67. Li L, Qian C, Zhao Y, et al. Enzyme kinetic characterization of microbe-produced urease for microbe-driven calcite mineralization. Reac Kinet Mech Cat, 2013, 108: 51–57

    Article  Google Scholar 

  68. Zheng T, Qian C, Su Y. Influences of different calcium sources on the early age cracks of self-healing cementitious mortar. Biochem Eng J, 2021, 166: 107849

    Article  Google Scholar 

  69. Chen Q, Su Y, Li M, et al. Calcium carbonate labeling for the characterization of self-healing cracks in cement-based materials. Mater Lett, 2021, 292: 129507

    Article  Google Scholar 

  70. Yi H, Qian C X. Synthesis and characterization of calcium carbonate nanoparticles via bacterial mineralization in steel slag comprising cementitious materials. Sci Adv Mater, 2020, 12: 760–768

    Article  Google Scholar 

  71. Rong H, Qian C X. Binding functions of microbe cement. Adv Eng Mater, 2015, 17: 334–340

    Article  Google Scholar 

  72. Qian C, Yu X, Wang X. A study on the cementation interface of biocement. Mater Charact, 2018, 136: 122–127

    Article  Google Scholar 

  73. Zhang X, Qian C. Engineering application of microbial self-healing concrete in lock channel wall. Mar Georesour Geotech, 2021, 1–8

  74. Qian C, Zheng T, Zhang X, et al. Application of microbial self-healing concrete: Case study. Constr Build Mater, 2021, 290: 123226

    Article  Google Scholar 

  75. Allied Market Research. Global Self-Healing Concrete Market-Industry Analysis and Forecast 2027. https://www.alliedmarketresearch.com/amp

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to ChunXiang Qian or TianWen Zheng.

Additional information

This work was supported by the National Natural Science Foundation of China (Grant No. 51738003).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Qian, C., Zheng, T. & Rui, Y. Living concrete with self-healing function on cracks attributed to inclusion of microorganisms: Theory, technology and engineering applications—A review. Sci. China Technol. Sci. 64, 2067–2083 (2021). https://doi.org/10.1007/s11431-021-1879-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11431-021-1879-6

Navigation