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Molecular insight into electron transfer properties of extracellular polymeric substances of electroactive bacteria by surface-enhanced Raman spectroscopy

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Abstract

Extracellular polymeric substances (EPS) extracted from electroactive bacteria show promising redox activity, but the electron transfer (ET) mechanism of the EPS has been rarely elucidated because of their structural complexity and lack of efficient methodologies. In this study, the charge transfer theory of surface-enhanced Raman spectroscopy (SERS) was applied to evaluate the redox properties of EPS adsorbed on Ni and Ag nanoparticles (NPs). These metal NPs were used to simultaneously magnify Raman signals and reduce/oxidize the redox moieties in EPS. As a result, the ET between EPS and metal NPs was evaluated through the changes in Raman signals. In this regard, we compared the redox activity of EPS extracted from two typical electroactive bacteria (Shewanella oneidensis and Geobacter sulfurreducens) and another two nonelectroactive strains (Escherichia coli and Bacillus subtilis). Electrochemical measurements show that the electroactive strains have higher redox capabilities than nonelectroactive strains. The SERS analysis shows that the porphyrin present in cytochrome c is the dominating redox moiety in the EPS of electroactive bacteria. The results of this study show that SERS with active metal substrates is a sensitive tool to probe the redox response of EPS, offering an opportunity to better understand the redox nature of biomolecules.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 51678162, 41877045, 21876032), the Guangzhou City Science-Technology Project (Grant No. 201707010250), and the Major Scientific Project of Guangdong University (Grant No. 2017KZDXM029).

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Authors and Affiliations

  1. Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou, 510006, China

    Bin Tan, ShaoFeng Zhou, Yi Wang, BeiPing Zhang & Yong Yuan

  2. Institute of Natural Medicine & Green Chemistry, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, China

    LiHua Zhou

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  1. Bin Tan
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  2. ShaoFeng Zhou
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  3. Yi Wang
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Correspondence to Yong Yuan.

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11431_2018_9437_MOESM1_ESM.doc

Molecular insight into electron transfer properties of extracellular polymeric substances of electroactive bacteria by surface-enhanced Raman spectroscopy

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Tan, B., Zhou, S., Wang, Y. et al. Molecular insight into electron transfer properties of extracellular polymeric substances of electroactive bacteria by surface-enhanced Raman spectroscopy. Sci. China Technol. Sci. 62, 1679–1687 (2019). https://doi.org/10.1007/s11431-018-9437-0

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