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Dopamine detection using a patch-clamp system on a planar microeletrode array electrodeposited by polypyrrole/graphene nanocomposites

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Abstract

To achieve a dopamine (DA) response with high sensitivity and high signal-to-noise ratio (S/N) with a patch-clamp system, polypyrrole/graphene (PPy/GR) nanocomposites were steadily electrodeposited by an electrochemical method on a planar microelectrode array (pMEA) fabricated by a standard micromachining process. The electrodeposition process was carried out by chronopotentimetry measurement scanning from 0.1 to 0.8 C/cm2 at the current of 2 mA; 0.5 C/cm2 was found to be optimal. The pMEA modified by PPy/GR at the 0.5 C/cm2 exhibits remarkable properties; for instance, the standard deviation (SD) decreases from 8.4614×10−11 to 5.62×10−11 A, reduced by 33.52%, and the sensitivity increases from 2566.88 to 76114.65 μAmMcm−2, 29.65 times higher than the bare Pt (platinum). A good linear relationship between the current and DA concentration in the range of 0.30 to 61.71 μm was obtained, with a correlation coefficient of 0.997. The sensor is meaningful for neuroscience research and the treatment of neurological diseases.

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Authors and Affiliations

  1. State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, China

    Li Wang, TingJun Jiang, YiLin Song, WenTao Shi & XinXia Cai

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Li Wang, TingJun Jiang & XinXia Cai

Authors
  1. Li Wang
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  2. TingJun Jiang
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  3. YiLin Song
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  4. WenTao Shi
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  5. XinXia Cai
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Correspondence to XinXia Cai.

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Wang, L., Jiang, T., Song, Y. et al. Dopamine detection using a patch-clamp system on a planar microeletrode array electrodeposited by polypyrrole/graphene nanocomposites. Sci. China Technol. Sci. 57, 288–292 (2014). https://doi.org/10.1007/s11431-014-5465-9

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  • DOI: https://doi.org/10.1007/s11431-014-5465-9

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