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References
Bernstein, B.E., Mikkelsen, T.S., Xie, X., Kamal, M., Huebert, D.J., Cuff, J., Fry, B., Meissner, A., Wernig, M., Plath, K., et al. (2006). A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell 125, 315–326.
Cruz-Molina, S., Respuela, P., Tebartz, C., Kolovos, P., Nikolic, M., Fueyo, R., van Ijcken, W.F.J., Grosveld, F., Frommolt, P., Bazzi, H., et al. (2017). PRC2 facilitates the regulatory topology required for poised enhancer function during pluripotent stem cell differentiation. Cell Stem Cell 20, 689–705.e9.
Dai, Z., Li, R., Hou, Y., Li, Q., Zhao, K., Li, T., Li, M.J., and Wu, X. (2021). Inducible CRISPRa screen identifies putative enhancers. J Genet Genomics 48, 917–927.
Geula, S., Moshitch-Moshkovitz, S., Dominissini, D., Mansour, A.A.F., Kol, N., Salmon-Divon, M., Hershkovitz, V., Peer, E., Mor, N., Manor, Y.S., et al. (2015). m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation. Science 347, 1002–1006.
Harlen, K.M., and Churchman, L.S. (2017). The code and beyond: transcription regulation by the RNA polymerase II carboxy-terminal domain. Nat Rev Mol Cell Biol 18, 263–273.
Hu, D., Garruss, A.S., Gao, X., Morgan, M.A., Cook, M., Smith, E.R., and Shilatifard, A. (2013). The Mll2 branch of the COMPASS family regulates bivalent promoters in mouse embryonic stem cells. Nat Struct Mol Biol 20, 1093–1097.
Hu, S., Huo, D., Yu, Z., Chen, Y., Liu, J., Liu, L., Wu, X., and Zhang, Y. (2020). ncHMR detector: a computational framework to systematically reveal non-classical functions of histone modification regulators. Genome Biol 21, 48.
Ma, C., Karwacki-Neisius, V., Tang, H., Li, W., Shi, Z., Hu, H., Xu, W., Wang, Z., Kong, L., Lv, R., et al. (2016). Nono, a bivalent domain factor, regulates Erk signaling and mouse embryonic stem cell pluripotency. Cell Rep 17, 997–1007.
Ngan, C.Y., Wong, C.H., Tjong, H., Wang, W., Goldfeder, R.L., Choi, C., He, H., Gong, L., Lin, J., Urban, B., et al. (2020). Chromatin interaction analyses elucidate the roles of PRC2-bound silencers in mouse development. Nat Genet 52, 264–272.
Potjewyd, F., Turner, A.M.W., Beri, J., Rectenwald, J. M., Norris-Drouin, J.L., Cholensky, S.H., Margolis, D. M., Pearce, K.H., Herring, L.E., and James, L.I. (2020). Degradation of polycomb repressive complex 2 with an EED-targeted bivalent chemical degrader. Cell Chem Biol 27, 47–56.e15.
Riising, E.M., Comet, I., Leblanc, B., Wu, X., Johansen, J.V., and Helin, K. (2014). Gene silencing triggers polycomb repressive complex 2 recruitment to CpG islands genome wide. Mol Cell 55, 347–360.
Schuettengruber, B., Bourbon, H.M., Di Croce, L., and Cavalli, G. (2017). Genome regulation by polycomb and trithorax: 70 years and counting. Cell 171, 34–57.
Tee, W.W., Shen, S.S., Oksuz, O., Narendra, V., and Reinberg, D. (2014). Erk1/2 activity promotes chromatin features and RNAPII phosphorylation at developmental promoters in mouse ESCs. Cell 156, 678–690.
Zhao, X., and Wu, X. (2021). Polycomb-group proteins in the initiation and progression of cancer. J Genet Genomics 48, 433–443.
Acknowledgement
This work was supported by the National Natural Science Foundation of China (32320103009, 31970579), and the National Youth Talent Support Program. We thank the Core Facility of Research Center of Basic Medical Sciences, high performance computing (HPC) platform at Tianjin Medical University for technical support. We thank Dr. Kristian Helin for the kind gift of Ezh2f/f-ERT2-Cre mESCs, Dr. Lingyi Chen (NKU) for generously providing the caERK expression construct.
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Gong, M., Yuan, Y., Dai, Z. et al. PRC2 primes bivalent genes for transcription induction independent of histone methyltransferase activity. Sci. China Life Sci. (2024). https://doi.org/10.1007/s11427-024-2573-2
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DOI: https://doi.org/10.1007/s11427-024-2573-2