Article PDF
Avoid common mistakes on your manuscript.
References
Li, G., Li, X., Zhuang, S., Wang, L., Zhu, Y., Chen, Y., Sun, W., Wu, Z., Zhou, Z., Chen, J., et al. (2022). Gene editing and its applications in biomedicine. Sci China Life Sci doi: https://doi.org/10.1007/s11427-021-2057-0.
Nakamura, M., Gao, Y., Dominguez, A.A., and Qi, L.S. (2021). CRISPR technologies for precise epigenome editing. Nat Cell Biol 23, 11–22.
Qi, L.S., Larson, M.H., Gilbert, L.A., Doudna, J.A., Weissman, J.S., Arkin, A.P., and Lim, W.A. (2013). Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell 152, 1173–1183.
Qiu, F., Xing, S., Xue, C., Liu, J., Chen, K., Chai, T., and Gao, C. (2021). Transient expression of a TaGRF4-TaGIF1 complex stimulates wheat regeneration and improves genome editing. Sci China Life Sci doi: https://doi.org/10.1007/s11427-021-1949-9.
Song, R., Wang, Y., Zheng, Q., Yao, J., Cao, C., Wang, Y., and Zhao, J. (2022). One-step base editing in multiple genes by direct embryo injection for pig trait improvement. Sci China Life Sci doi: https://doi.org/10.1007/s11427-021-2013-8.
Tang, S., Yang, C., Wang, D., Deng, X., Cao, X., and Song, X. (2021). Targeted DNA demethylation produces heritable epialleles in rice. Sci China Life Sci doi: https://doi.org/10.1007/s11427-021-1974-7.
Yin, S., Ma, L., Shao, T., Zhang, M., Guan, Y., Wang, L., Hu, Y., Chen, X., Han, H., Shen, N., et al. (2020). Enhanced genome editing to ameliorate a genetic metabolic liver disease through co-delivery of adeno-associated virus receptor. Sci China Life Sci doi: https://doi.org/10.1007/s11427-020-1744-6.
Zhou, Y., Zhu, S., Cai, C., Yuan, P., Li, C., Huang, Y., and Wei, W. (2014). High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature 509, 487–491.
Zhu, S., Liu, Y., Zhou, Z., Zhang, Z., Xiao, X., Liu, Z., Chen, A., Dong, X., Tian, F., Chen, S., et al. (2021). Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry. Sci China Life Sci doi: https://doi.org/10.1007/s11427-021-1990-5.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Compliance and ethics
The authors declare that they have no conflict of interest.
Wensheng Wei: Professor of Biomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, and the School of Life Sciences at Peking University; and the director of Peking University Genome Editing Research Center. The research of Wei group is mainly focused on the development of eukaryotic gene editing tools, with the emphasis on the high-throughput functional genomics and gene therapy. The combination of forward and reverse genetic means are employed, often in a high-throughput fashion, for the understanding of the molecular mechanisms underlying human diseases, including cancer and infection.
Caixia Gao is a Principal Investigator at the Institute of Genetics and Developmental Biology (IGDB), Chinese Academy of Sciences. Prior to joining IGDB in 2009, she served as a Research Scientist at DLF’s biotechnology group in Denmark, where she worked on plant genetic transformation and molecular biology. She completed her Ph.D. from the China Agricultural University in Beijing. Her research group focuses on developing precision genome editing technologies and applying their use to create new agricultural crops of the future.
Rights and permissions
About this article
Cite this article
Wei, W., Gao, C. Gene editing: from technologies to applications in research and beyond. Sci. China Life Sci. 65, 657–659 (2022). https://doi.org/10.1007/s11427-022-2087-5
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11427-022-2087-5