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Individual variation in buffalo somatic cell cloning efficiency is related to glycolytic metabolism

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Abstract

Mammalian individuals differ in their somatic cell cloning efficiency, but the mechanisms leading to this variation is poorly understood. Here we found that high cloning efficiency buffalo fetal fibroblasts (BFFs) displayed robust energy metabolism, looser chromatin structure, high H3K9 acetylation and low heterochromatin protein 1α (HP1α) expression. High cloning efficiency BFFs had more H3K9ac regions near to the upstream of glycolysis genes by ChIP-seq, and involved more openness loci related to glycolysis genes through ATAC-seq. The expression of these glycolysis genes was also found to be higher in high cloning efficiency BFFs by qRT-PCR. Two key enzymes of glycolysis, PDKs and LDH, were confirmed to be associated with histone acetylation and chromatin openness of BFFs. Treatment of low cloning efficiency BFFs with PS48 (activator of PDK1) resulted in an increase in the intracellular lactate production and H3K9 acetylation, decrease in histone deacetylase activity and HP1α expression, less condensed chromatin structure and more cloning embryos developing to blastocysts. These results indicate that the cloning efficiency of buffalo somatic cells is associated with their glycolytic metabolism and chromatin structure, and can be improved by increasing glycolytic metabolism.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31772597, 31972996, 31902125), and Guangxi Natural Science Foundation (2017GXNSFAA198311). We thank Dr Tengfei Zhu (Annoroad Gene Technology) for his helpful assistance on bioinformatics analysis.

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, 530005, China

    Chan Luo, Zhiqiang Wang, Jinling Wang, Feng Yun, Fenghua Lu, Jiayuan Fu, Qingyou Liu & Deshun Shi

  2. College of Animal Science and Technology, Guangxi University, Nanning, 530005, China

    Chan Luo, Jinling Wang, Feng Yun, Fenghua Lu, Jiayuan Fu, Qingyou Liu & Deshun Shi

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  1. Chan Luo
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  2. Zhiqiang Wang
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Correspondence to Deshun Shi.

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Luo, C., Wang, Z., Wang, J. et al. Individual variation in buffalo somatic cell cloning efficiency is related to glycolytic metabolism. Sci. China Life Sci. 65, 2076–2092 (2022). https://doi.org/10.1007/s11427-021-2039-6

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