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Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China

Abstract

The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls; the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31521005, 31672593), the Laboratory for Lingnan Modern Agriculture Project (NT2021007), the National Key Research and Development Program of China (2016YFD0500201), and the China Agriculture Research System (CARS-41G12). We thank the authors and submitting laboratories of the sequences from the GISAID EpiFlu Database.

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Correspondence to Guohua Deng or Hualan Chen.

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Cui, P., Zeng, X., Li, X. et al. Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China. Sci. China Life Sci. 65, 795–808 (2022). https://doi.org/10.1007/s11427-021-2025-y

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Keywords

  • avian influenza virus
  • H5N8
  • evolution
  • pathogenicity
  • antigenicity
  • vaccine
  • protective efficacy