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Potential role of Lactobacillus plantarum in colitis induced by dextran sulfate sodium through altering gut microbiota and host metabolism in murine model

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Abstract

Inflammatory bowel disease (IBD) is a chronic lifelong disease characterized by inflammation of the gastrointestinal tract. Although more and more treatment options serve IBD, there is still no cure. It is important to find an effective treatment for IBD. This study aims to investigate whether Lactobacillus plantarum (L. plantarum) could alleviate colitis induced by dextran sulfate sodium (DSS). Following the DSS challenge, L. plantarum on DSS-mediated inflammatory colon lesions in mice, and L. plantarum therapy heightened the relative abundance of the colon-resident Actinobacteria. Analysis of serum metabolomics also indicated that the content of MG (18:4 (6Z, 9Z, 12Z, 15Z)/0:0/0:0) was increased in response to L. plantarum therapy, and this was also the case for indolepyruvate and 1-hydroxyibuprofen. However, 13-oxooctadecadienoic acid (13-oxoODE) and indolylacryloylglycine content fell following the DSS challenge. Based on these results, the study elucidates the mitigatory effects of L. plantarum in colitis, which depend on its regulation of the colonic microbial community and its modification of serum metabolites. The results revealed that L. plantarum mitigated inflammatory colon lesions, reprogrammed the microbial community and altered the level of serum metabolites in a murine model challenged with DSS. The study may present a potential therapeutic strategy for colitis.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (31772642, 31672457), Hunan Provincial Science and Technology Department (2019TP2004, 2017NK2322, 2016WK2008, 2016TP2005), Double first-class construction project of Hunan Agricultural University (SYL201802003), and China Postdoctoral Science Foundation (2018M632963, 2019T120705).

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  1. College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Provincial Engineering Research Center of Applied Microbial Resources Development for Livestock and Poultry, Changsha, 410128, China

    Sujuan Ding, Wenxin Yan, Jun Fang, Hongmei Jiang & Gang Liu

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  1. Sujuan Ding
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  2. Wenxin Yan
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  3. Jun Fang
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  4. Hongmei Jiang
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Correspondence to Gang Liu.

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The author(s) declare that they have no conflict of interest. All aspects of this research were conducted in accordance with the Chinese Guidelines for Animal Welfare and Experimental Protocols. The Hunan Agricultural University’s Animal Ethics Committee granted approval for the animal procedures used in this study.

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Ding, S., Yan, W., Fang, J. et al. Potential role of Lactobacillus plantarum in colitis induced by dextran sulfate sodium through altering gut microbiota and host metabolism in murine model. Sci. China Life Sci. 64, 1906–1916 (2021). https://doi.org/10.1007/s11427-020-1835-4

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