Abstract
Mural cells (MCs) wrap around the endothelium, and participate in the development and homeostasis of vasculature. MCs have been reported as heterogeneous population morphologically and functionally. However, the transcriptional heterogeneity of MCs was rarely studied. In this study, we illustrated the transcriptional heterogeneity of MCs with different perspectives by using publicly available single-cell dataset GSE109774. Specifically, MCs are transcriptionally different from other cell types, and ligand-receptor interactions of different cells with MCs vary. Re-clustering of MCs identified five distinct subclusters. The heterogeneity of MCs in tissues was reflected by MC coverage, various distribution of MC subclusters, and ligand-receptor interactions of MCs and parenchymal cells. The transcriptomic diversity of MCs revealed in this article will help facilitate further research into MCs.
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Acknowledgements
The authors acknowledge with thanks the R script used for cellphoneDB analysis provided by Dr N. C. Henderson. This work was supported in part by the National Key Research and Development Program of China (2019YFA0802700, 2017YFA0103700) and the National Natural Science Foundation of China (91739301, 91339205, 81130005).
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Guan, YN., Li, Y., Roosan, M. et al. Single-cell transcriptomics of murine mural cells reveals cellular heterogeneity. Sci. China Life Sci. 64, 1077–1086 (2021). https://doi.org/10.1007/s11427-020-1823-2
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DOI: https://doi.org/10.1007/s11427-020-1823-2
- mural cells
- single-cell analysis
- heterogeneity