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Arabidopsis AIP1-1 regulates the organization of apical actin filaments by promoting their turnover in pollen tubes

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Abstract

Apical actin filaments are highly dynamic structures that are crucial for rapid pollen tube growth, but the mechanisms regulating their dynamics and spatial organization remain incompletely understood. We here identify that AtAIP1-1 is important for regulating the turnover and organization of apical actin filaments in pollen tubes. AtAIP1-1 is distributed uniformly in the pollen tube and loss of function of AtAIP1-1 affects the organization of the actin cytoskeleton in the pollen tube. Specifically, actin filaments became disorganized within the apical region of aip1-1 pollen tubes. Consistent with the role of apical actin filaments in spatially restricting vesicles in pollen tubes, the apical region occupied by vesicles becomes enlarged in aip1-1 pollen tubes compared to WT. Using ADF1 as a representative actin-depolymerizing factor, we demonstrate that AtAIP1-1 enhances ADF1-mediated actin depolymerization and filament severing in vitro, although AtAIP1-1 alone does not have an obvious effect on actin assembly and disassembly. The dynamics of apical actin filaments are reduced in aip1-1 pollen tubes compared to WT. Our study suggests that AtAIP1-1 works together with ADF to act as a module in regulating the dynamics of apical actin filaments to facilitate the construction of the unique “apical actin structure” in the pollen tube.

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Acknowledgements

We thank Yan Zhang (Shandong Agricultural University) for providing the transgenic lines expressing Lat52:YFP-RabA4b. This work was supported by a grant from the National Natural Science Foundation of China (31671390) and funding from the Tsinghua-Peking Joint Center for Life Sciences.

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Authors and Affiliations

  1. Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Min Diao & Xin Li

  2. Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China

    Min Diao & Shanjin Huang

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Xin Li

  4. iHuman Institute, Shanghai Tech University, Shanghai, 201210, China

    Min Diao

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  1. Min Diao
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Correspondence to Shanjin Huang.

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Diao, M., Li, X. & Huang, S. Arabidopsis AIP1-1 regulates the organization of apical actin filaments by promoting their turnover in pollen tubes. Sci. China Life Sci. 63, 239–250 (2020). https://doi.org/10.1007/s11427-019-9532-0

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