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Mating patterns and pollen dispersal in a Japanese larch (Larix kaempferi) clonal seed orchard: a case study

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Abstract

Pollination dynamics highly determines the genetic quality of seed orchard crops. However, there is less research about the effect of mating patterns on seed productivity of orchard crops. So far, clonal seed orchards have been producing genetically improved seedlings used for most Japanese larch (Larix kaempferi (Lamb.) Carr.) plantations in China. In the present study, a total of 17 highly variable simple sequence repeat (SSR) markers were used for genotyping a progeny trial population consisting of 647 open-pollinated progenies germinated from seeds which were collected from 63 maternal clones with 140 potential paternal clones in a Japanese larch clonal seed orchard in China. Paternity analysis was used in the present case study in order to evaluate the level of paternal gametic contribution, estimate pollen contamination and selfing rates, and investigate pollination patterns, pollen dispersal patterns and the impact of mating patterns on seed productivity of orchard crops. We observed 93.7% of the success rate of the parental assignment, unequal paternal gametic contribution (0–12.4%) with 6.3% of the progenies derived from pollen contamination or unsampled pollen donors, and absence of evidence for selfing. We also found that pollination rate highly depended on the distance between pollen donors and maternal parents, the majority of the identified crossing (65.7%) occurred between clones within a 150-m radius, and large variations in growth performance existed among the paternal half-siblings. Progeny growth performance (diameter at breast (DBH) and height (HGT)) was measured at Age-20 in order to investigate the impact of mating patterns on timber production of orchard crops. As either the paternal or maternal, two clones (i. e., clones Z38 and Z62) were identified to have produced progenies with higher average stem volume breeding values than that of all of the progenies. Specifically, the genetic gains for volume were 3.53% for the two clones as paternal parents, and 8.26% as the maternal parents at Age-20. Thus, both elite clones were ideal candidates for the construction of next-generation clonal seed orchards due to their synchronous reproductive phenology with greater crossing rate and higher genetic gain. These results improved the pedigree information to provide solid evidence of mating patterns for future design and effective management of seed orchards and for the development of viable long-term breeding strategies for other coniferous species.

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References

  • Aronen, T., Nikkanen, T., Harju, A., Tiimonen, H., and Häggman, H. (2002). Pollen competition and seed-siring success in Picea abies. TAG Theor Appl Genets 104, 638–642.

    Article  Google Scholar 

  • Bai, W.N., Zeng, Y.F., and Zhang, D.Y. (2007). Mating patterns and pollen dispersal in a heterodichogamous tree,Juglans mandshurica (Juglandaceae). New Phytologist 176, 699–707.

    Article  PubMed  Google Scholar 

  • Bilgen, B.B., and Kaya, N. (2015). Chloroplast DNA variation and pollen contamination in a Pinus brutia Ten. clonal seed orchard: implication for progeny performance in plantations. Turk J Agric For 38, 540–549.

    Article  Google Scholar 

  • Burczyk, J., Nikkanen, T., and Lewandowski, A. (1997). Evidence of an unbalanced mating pattern in a seed orchard composed of two larch species. Silvae Genet 2, 176–180.

    Google Scholar 

  • Canchignia-Martínez, H.F., Hernández-Delgado, S., González-Paz, M., Motte, E., and Mayek-Pérez, N. (2007). Genetic relationships among Schizolobium parahybum (Vell.) Blake (Leguminosae) ecotypes from Ecuador and other countries. Silvae Genet 5, 214–221.

    Article  Google Scholar 

  • Chen, X.B., Xie, Y.H., and Sun, X.M. (2015). Development and characterization of polymorphic genic-SSR markers in Larix kaempferi. Molecules 20, 6060–6067.

    Article  PubMed  CAS  Google Scholar 

  • Chaloupková, K., Stejskal, J., El-Kassaby, Y.A., and Lstiburek M. (2016). Optimum neighborhood seed orchard design. Tree Genet Genomes 12, 105.

    Article  Google Scholar 

  • Codesido, V., Merlo, E., and Fernández-López, J. (2005). Variation in reproductive phenology in a Pinus radiata D. Don seed orchard in northern Spain. Silvae Genet 1995, 4–5.

    Google Scholar 

  • Doyle, J. (1935). Pollination in Tsuga, Cedrus, Pseudotsuga, and Larix. Sci Proc R Dublin Soc, 191–204.

    Google Scholar 

  • El-Kassaby, Y.A., and Lindgren, D. (2007). Increasing the efficiency of breeding without breeding through phenotypic pre-selection in open pollinated progenies. In Joint Meeting of the South. For. Tree Improve. Conf. and the Western For. Genetics Association, Galveston, Texas. pp. 15–19.

    Google Scholar 

  • El-Kassaby, Y.A., and Lstiburek, M. (2009). Breeding without breeding. Genet Res 91, 111–120.

    Article  Google Scholar 

  • El-Kassaby, Y.A., Stoehr, M.U., Reid, D., Walsh, C.G., and Lee, T.E. (2007). Clonal-row versus random seed orchard designs: interior spruce mating system evaluation. Can J For Res 37, 690–696.

    Article  CAS  Google Scholar 

  • Fernandes, L., Rocheta, M., Cordeiro, J., Pereira, S., Gerber, S., Oliveira, M.M., and Ribeiro, M.M. (2008). Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard. Ann For Sci 65, 706–706.

    Article  Google Scholar 

  • Funda, T., Chen, C.C., Liewlaksaneeyanawin, C., Kenawy, A.M.A., and El-Kassaby, Y.A. (2008). Pedigree and mating system analyses in a western larch (Larix occidentalis Nutt.) experimental population. Ann For Sci 65, 705–705.

    Article  Google Scholar 

  • Funda, T., Wennström, U., Almqvist, C., Andersson Gull, B., and Wang, X. R. (2016). Mating dynamics of Scots pine in isolation tents. Tree Genets Genomes 12, 112.

    Article  Google Scholar 

  • Funda, T., Wennström, U., Almqvist, C., Almqvist, C., Torimaru, T., Gull, B.A., and Wang, X.R. (2015). Low rates of pollen contamination in a Scots pine seed orchard in Sweden: the exception or the norm? Scand J Forest Res 30, 573–586.

    Article  Google Scholar 

  • Gonzaga, J.M.S., Manoel, R.O., Sousa, A.C.B., Souza, A.P., Moraes, M.L. T., and Sebbenn, A.M. (2016). Pollen contamination and nonrandom mating in a Eucalyptus camaldulensis Dehnh seedling seed orchard. Silvae Genetica 65, https://doi.org/10.1515/sg-2016-0001.

    Google Scholar 

  • Grattapaglia, D., do Amaral Diener, P.S., and dos Santos, G.A. (2014). Performance of microsatellites for parentage assignment following mass controlled pollination in a clonal seed orchard of loblolly pine (Pinus taeda L.). Tree Genets Genomes 10, 1631–1643.

    Article  Google Scholar 

  • Hadfield, J.D., Richardson, D.S., and Burke, T. (2006). Towards unbiased parentage assignment: combining genetic, behavioural and spatial data in a Bayesian framework. Mol Ecol 15, 3715–3730.

    Article  PubMed  CAS  Google Scholar 

  • Hansen, O.K. (2008). Mating patterns, genetic composition and diversity levels in two seed orchards with few clones—Impact on planting crop. For Ecol Manage 256, 1167–1177.

    Article  Google Scholar 

  • Hansen, O.K., and McKinney, L.V. (2010). Establishment of a quasi-field trial in Abies nordmanniana—test of a new approach to forest tree breeding. Tree Genets Genomes 6, 345–355.

    Article  Google Scholar 

  • Hasegawa, Y., Suyama, Y., and Seiwa, K. (2009). Pollen donor composition during the early phases of reproduction revealed by DNA genotyping of pollen grains and seeds of Castanea crenata. New Phytologist 182, 994–1002.

    Article  PubMed  CAS  Google Scholar 

  • Isoda, K., and Watanabe, A. (2006). Isolation and characterization of microsatellite loci from Larix kaempferi. Mol Ecol Notes 6, 664–666.

    Article  CAS  Google Scholar 

  • Jones, A.G., Small, C.M., Paczolt, K.A., and Ratterman, N.L. (2010). A practical guide to methods of parentage analysis. Mol Ecol Resources 10, 6–30.

    Article  Google Scholar 

  • Kalinowski, S.T., Taper, M.L., and Marshall, T.C. (2007). Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment. Mol Ecol 16, 1099–1106.

    Article  PubMed  Google Scholar 

  • Kess, T., and El-Kassaby, Y.A. (2015). Estimates of pollen contamination and selfing in a coastal Douglas-fir seed orchard. Scand J For Res 4, 266–275.

    Google Scholar 

  • Kaya, N., and IsIk, K. (2010). Genetic identification of clones and the genetic structure of seed crops in a Pinus brutia seed orchard. Turk J Agric For 34, 127–134.

    CAS  Google Scholar 

  • Lai, M., Sun, X., Chen, D., Xie, Y., and Zhang, S. (2014). Age-related trends in genetic parameters for Larix kaempferi and their implications for early selection. BMC Genet 15, S10.

    Article  PubMed  PubMed Central  Google Scholar 

  • Lewandowski, A., Burczyk, J., and Mejnartowicz, L. (1991). Genetic structure and the mating system in an old stand of Polish larch. Silvae Genet 2, 75–79.

    Google Scholar 

  • Li, W., Wang, X., and Li, Y. (2011). Stability in and correlation between factors influencing genetic quality of seed lots in seed orchard of Pinus tabuliformis Carr. over a 12-year span. PLoS ONE 8, e23544.

    Article  CAS  Google Scholar 

  • Lstiburek, M., Stejskal, J., Misevicius, A., Korecký, J., and El-Kassaby, Y. A. (2015). Expansion of the minimum-inbreeding seed orchard design to operational scale. Tree Genet Genomes 11, 1–8.

    Article  Google Scholar 

  • Millar, M.A., Byrne, M., Nuberg, I., and Sedgley, M. (2008). High outcrossing and random pollen dispersal in a planted stand of Acacia saligna subsp. saligna revealed by paternity analysis using microsatellites. Tree Genets Genomes 4, 367–377.

    Article  Google Scholar 

  • Moriguchi, Y., Tani, N., Itoo, S., Kanehira, F., Tanaka, K., Yomogida, H., Taira, H., and Tsumura, Y. (2005). Gene flow and mating system in five Cryptomeria japonica D. Don seed orchards as revealed by analysis of microsatellite markers. Tree Genet Genomes 4, 174–183.

    Article  Google Scholar 

  • Moriguchi, Y., Yamazaki, Y., Taira, H., and Tsumura, Y. (2010). Mating patterns in an indoor miniature Cryptomeria japonica seed orchard as revealed by microsatellite markers. New Fors 39, 261–273.

    Article  Google Scholar 

  • Nielsen, U.B., and Hansen, O.K. (2012). Genetic worth and diversity across 18 years in a Nordmann fir clonal seed orchard. Ann For Sci 69, 69–80.

    Article  Google Scholar 

  • Nikkanen, T., Aronen, T., Häggman, H., and Venäläinen, M. (2000). Variation in pollen viability among Picea abies genotypes—potential for unequal paternal success. TAG Theor Appl Genets 101, 511–518.

    Article  Google Scholar 

  • Nishimura, M., and Setoguchi, H. (2011). Homogeneous genetic structure and variation in tree architecture of Larix kaempferi along altitudinal gradients on Mt. Fuji. J Plant Res 124, 253–263.

    Article  PubMed  Google Scholar 

  • Roberds, J.H., Friedman, S.T., and El-Kassaby, Y.A. (1991). Effective number of pollen parents in clonal seed orchards. Theoret Appl Genets 82, 313–320.

    Article  CAS  Google Scholar 

  • Robledo-Arnuncio, J.J., and Gil, L. (2005). Patterns of pollen dispersal in a small population of Pinus sylvestris L. revealed by total-exclusion paternity analysis. Heredity 1, 13–22.

    Article  Google Scholar 

  • Saro, I., Robledo-Arnuncio, J.J., González-Pérez, M.A., and Sosa, P.A. (2014). Patterns of pollen dispersal in a small population of the Canarian endemic palm (Phoenix canariensis). Heredity 113, 215–223.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Schoen, D.J., and Stewart, S.C. (1986). Variation in male reproductive investment and male reproductive success in white spruce. Evolution 40, 1109–1120.

    Article  PubMed  Google Scholar 

  • Silva, P.I.T., Martins, A.M., Gouvea, E.G., Pessoa-Filho, M., and Ferreira, M.E. (2013). Development and validation of microsatellite markers for Brachiaria ruziziensis obtained by partial genome assembly of Illumina single-end reads. BMC Genomics 14, 17.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Slobodník, B. (2002). Pollination success and full seed percentage in European larch (Larix decidua MILL.). J For Sci 1, 271–280.

    Google Scholar 

  • Slobodník, B., and Guttenberger, H. (2005). Zygotic embryogenesis and empty seed formation in European larch (Larix decidua Mill.). Ann For Sci 2, 129–134.

    Article  Google Scholar 

  • Sønstebø, J.H., Tollefsrud, M.M., Myking, T., Steffenrem, A., Nilsen, A.E., Edvardsen, Ø.M., Johnskås, O.R., and El-Kassaby, Y.A. (2018). Genetic diversity of Norway spruce (Picea abies (L.) Karst.) seed orchard crops: Effects of number of parents, seed year, and pollen contamination. For Ecol Manage 411, 132–141.

    Article  Google Scholar 

  • Stoehr, M., Mehl, H., Nicholson, G., Pieper, G., and Newton, C. (2006). Evaluating supplemental mass pollination efficacy in a lodgepole pine orchard in British Columbia using chloroplast DNA markers. New For 31, 83–90.

    Article  Google Scholar 

  • Stoehr, M.U., and El-Kassaby, Y.A. (1997). Levels of genetic diversity at different stages of the domestication cycle of interior spruce in British Columbia. Theoret Appl Genets 94, 83–90.

    Article  CAS  Google Scholar 

  • Summers, K., and Amos, W. (1997). Behavioral, ecological, and molecular genetic analyses of reproductive strategies in the Amazonian dart-poison frog, Dendrobates ventrimaculatus. Behaval Ecol 8, 260–267.

    Article  Google Scholar 

  • Sun, X., Zhang, S., Zhou, D., and Wang, X. (2008). Phenological variation of Larix species and their intra-species and inter-species hybrid families and early selection (in Chinese). Sci Silvae Sin 1, 77–84.

    Google Scholar 

  • Sun, X.M., and Yang, X.Y. (2011). Applications and analysis of methods for breeding value prediction in forest trees (in Chinese). J Beijing For Univ 2, 65–71.

    Google Scholar 

  • Torimaru, T., Wennström, U., Andersson, B., Almqvist, C., and Wang, X.R. (2013). Reduction of pollen contamination in Scots pine seed orchard crop by tent isolation. Scandinavian J For Res 28, 715–723.

    Article  Google Scholar 

  • Wang, Y.C., Dong, X.G., Wang, X.S., and Ma, H. (2000). Study on seed production and fruiting law of seed orchard in Larix kaempferi (Lamb.) Carr. (in Chinese). Sci Silvae Sin 2, 53–59.

    Google Scholar 

  • White, T.L., Adams, W.T., and Neale, D.B. (2007). Forest Genetics, 1st ed. CABI.

    Book  Google Scholar 

  • Wei, Z., Qu, Z., Hou, C., Liu, Y., Zhang, L., Yang, C., and Wei, H. (2015). Genetic diversity and paternal analysis of open-pollinated progenies of Larix olgensis seed orchard. J Nat Sci 1, e19.

    Google Scholar 

  • Yang, X., Sun, X., and Zhang, S. (2011). Short note: Development of six EST-SSR markers in Larch. Silvae Genet 3–4, 161–163.

    Article  Google Scholar 

  • Yeh, F.C., and Boyle, J. (1997). POPGENE, the user-friendly shareware for population genetic analysis. Mol Biol Biotechnol, 724–731.

    Google Scholar 

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Acknowledgements

This work was supported by the Forestry Industry Research Special Funds for Public Welfare Projects (201504104). We gratefully thank Wusheng Liu for his guidance and thorough review of the manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China

    Xingbin Chen, Xiaomei Sun, Leiming Dong & Shougong Zhang

  2. Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China

    Xingbin Chen, Xiaomei Sun, Leiming Dong & Shougong Zhang

  3. Jiangxi Provincial Key Lab for Plant Biotechnology, Jiangxi Academy of Forestry, Nanchang, 330013, China

    Xingbin Chen

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  1. Xingbin Chen
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Correspondence to Shougong Zhang.

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Chen, X., Sun, X., Dong, L. et al. Mating patterns and pollen dispersal in a Japanese larch (Larix kaempferi) clonal seed orchard: a case study. Sci. China Life Sci. 61, 1011–1023 (2018). https://doi.org/10.1007/s11427-018-9305-7

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