This is a preview of subscription content, log in via an institution to check access.
References
Anastassiadis, K., Fu, J., Patsch, C., Hu, S., Weidlich, S., Duerschke, K., Buchholz, F., Edenhofer, F., and Stewart, A.F. (2009). Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Dis Model Mech 2, 508–515.
Attanasio, C., Nord, A.S., Zhu, Y., Blow, M.J., Li, Z., Liberton, D.K., Morrison, H., Plajzer-Frick, I., Holt, A., Hosseini, R., et al. (2013). Fine tuning of craniofacial morphology by distant-acting enhancers. Science 342, 1241006–1241006.
Awatramani, R., Soriano, P., Rodriguez, C., Mai, J.J., and Dymecki, S.M. (2003). Cryptic boundaries in roof plate and choroid plexus identified by intersectional gene activation. Nat Genet 35, 70–75.
Ayadi, A., Birling, M.C., Bottomley, J., Bussell, J., Fuchs, H., Fray, M., Gailus-Durner, V., Greenaway, S., Houghton, R., Karp, N., et al. (2012). Mouse large-scale phenotyping initiatives: overview of the European Mouse Disease Clinic (EUMODIC) and of the Wellcome Trust Sanger Institute Mouse Genetics Project. Mamm Genome 23, 600–610.
Barriga, E.H., Trainor, P.A., Bronner, M., and Mayor, R. (2015). Animal models for studying neural crest development: is the mouse different? Development 142, 1555–1560.
Branda, C.S., and Dymecki, S.M. (2004). Talking about a revolution. Dev Cell 6, 7–28.
Brewer, J.R., Molotkov, A., Mazot, P., Hoch, R.V., and Soriano, P. (2015). Fgfr1 regulates development through the combinatorial use of signaling proteins. Genes Dev 29, 1863–1874.
Danielian, P.S., Muccino, D., Rowitch, D.H., Michael, S.K., and McMahon, A.P. (1998). Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase. Curr Biol 8, 1323–S2.
Gerfen, C.R., Paletzki, R., and Heintz, N. (2013). GENSAT BAC Crerecombinase driver lines to study the functional organization of cerebral cortical and basal ganglia circuits. Neuron 80, 1368–1383.
Hermann, M., Stillhard, P., Wildner, H., Seruggia, D., Kapp, V., Sánchez-Iranzo, H., Mercader, N., Montoliu, L., Zeilhofer, H.U., and Pelczar, P. (2014). Binary recombinase systems for high-resolution conditional mutagenesis. Nucleic Acids Res 42, 3894–3907.
Lao, Z., Raju, G.P., Bai, C.B., and Joyner, A.L. (2012). MASTR: A technique for mosaic mutant analysis with spatial and temporal control of recombination using conditional floxed alleles in mice. Cell Rep 2, 386–396.
Legué, E., and Joyner, A.L. (2010). Genetic fate mapping using site-specific recombinases. Methods Enzymol 477, 153–181.
Lewis, A.E., Vasudevan, H.N., O’Neill, A.K., Soriano, P., and Bush, J.O. (2013). The widely used Wnt1-Cre transgene causes developmental phenotypes by ectopic activation of Wnt signaling. Dev Biol 379, 229–234.
Prescott, S.L., Srinivasan, R., Marchetto, M.C., Grishina, I., Narvaiza, I., Selleri, L., Gage, F.H., Swigut, T., and Wysocka, J. (2015). Enhancer divergence and cis-regulatory evolution in the human and chimp neural crest. Cell 163, 68–83.
Raymond, C.S., and Soriano, P. (2007). High-efficiency FLP and FC31 site-specific recombination in mammalian cells. PLoS ONE 2, e162.
Tallquist, M.D., and Soriano, P. (2003). Cell autonomous requirement for PDGFRalpha in populations of cranial and cardiac neural crest cells. Development 130, 507–518.
Uslu, V.V., Petretich, M., Ruf, S., Langenfeld, K., Fonseca, N.A., Marioni, J.C., and Spitz, F. (2014). Long-range enhancers regulating Myc expression are required for normal facial morphogenesis. Nat Genet 46, 753–758.
Acknowledgements
I thank members of my laboratory for helpful discussions and critical reading of the manuscript. I apologize to colleagues whose work I have been unable to cite due to space limitations. Work in my laboratory is supported by grants RO1 DE022363 and RO1 DE022778 from the National Institute of Dental and Craniofacial Research.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Soriano, P. Intersectional gene inactivation: there is more to conditional mutagenesis than Cre. Sci. China Life Sci. 61, 1115–1117 (2018). https://doi.org/10.1007/s11427-018-9291-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11427-018-9291-2