Abstract
Chlorophyll a and β-carotene play an important role in harvesting light energy, which is used to drive photosynthesis in plants. In this study, terahertz (THz) and visible range spectra of chlorophyll a and β-carotene and their changes under light treatment were investigated. The results show that the all THz transmission and absorption spectra of chlorophyll a and β-carotene changed upon light treatment, with the maximum changes at 15 min of illumination indicating the greatest changes of the collective vibrational mode of chlorophyll a and β-carotene. The absorption spectra of chlorophyll a in the visible light region decreased upon light treatment, signifying the degradation of chlorophyll a molecules. It can be inferred from these results that the THz spectra are very sensitive in monitoring the changes of the collective vibrational mode, despite the absence of changes in molecular configuration. The THz spectra can therefore be used to monitor the decomposing process of biological macromolecules; however, visible absorption spectra can only be used to monitor the breakdown extent of biological macromolecules.
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References
Allahverdieva, Y., and Aro, E.M. (2012). Photosynthetic responses of plants to excess light: mechanisms and conditions for photoinhibition, excess energy dissipation and repair. In Photosynthesis: Plastid Biology, Energy Conversion and Carbon Assimilation. Advances in Photosynthesis and Respiration, J.J. Eaton-Rye, B.C. Tripathy, and T.D. Sharkey, eds. (Dordrecht: Springer), pp. 275–297.
Barber, J. (1998). Photosystem two. Biochim Biophys Acta 1365, 269–277.
Björkman, O. (1987). High-irradiance stress in higher plants and interaction with other stress factors. In Progress in Photosynthesis Research, J. Biggins, ed. (Dordrecht: Springer), pp. 11–18.
Cheng, P. (2006). The Contrast formation in optical microscopy. In Handbook of Biological Confocal Microscopy, B.P., James, ed. (New York: Springer), pp. 162–206.
Domonkos, I., Kis, M., and Gombos, Z. (2013). Carotenoids, versatile components of oxygenic photosynthesis. Prog Lipid Res 52, 539–561.
Hörtensteiner, S. (2006). Chlorophyll degradation during senescence. Annu Rev Plant Biol 57, 55–77.
Kingsley, R.S., Shelley, J., and James, E.B. (2003). photosynthesis. In Introductory Plant Biology, R.S. Kingsley, J. Shelley, and E.B. James, ed. (New York: McGraw-Hill Companies), pp. 172–186.
Korter, T.M., Balu, R., Campbell, M.B., Beard, M.C., Gregurick, S.K., and Heilweil, E.J. (2005). Terahertz spectroscopy of solid serine and cysteine. Chem Phys Lett 418, 65–70.
Kuczynska, P., Jemiola-Rzeminska, M., and Strzalka, K. (2015). Photosynthetic Pigments in Diatoms. Mar Drugs 13, 5847–5881.
Zhang, L., Zuo, J., and Zhang, C. (2014). Observation of terahertz spectra of all-trans β-Carotene. Spectrosc Spect Anal 34, 405–406.
Markelz, A., Whitmire, S., Hillebrecht, J., and Birge, R. (2002). THz time domain spectroscopy of biomolecular conformational modes. Phys Med Bio 21, 3797–3805.
May, V., and Kühn, O. (2011). Charge and Energy Transfer Dynamics in Molecular Systems (Weinheim: Wiley-VCH Verlag GmbH & Co.).
Ohad, I., Berg, A., Berkowicz, S.M., Kaplan, A., and Keren, N. (2011). Photoinactivation of photosystem II: is there more than one way to skin a cat? Physiol Plantarum 142, 79–86.
Öquist, G. (1988). Stress and adaptation in photosynthesis. In Light in Biology and Medicine, R.H. Douglas, J. Moan, and F.D. Acqua, ed. (New York: Plenum Press), pp. 433–440.
Tyystjärvi, E. (2013). Photoinhibition of photosystem II.Int Rev Cel Mol Bio 300, 243–302.
Walther, M., Plochocka, P., Fischer, B., Helm, H., and Jepsen, P. Uhd. (2002). Collective vibrational modes in biological molecules investigated by terahertz timedomain spectroscopy. Biopolymers 67, 310–313.
Whitmire, S.E., Wolpert, D., Markelt, A.G., Hillebrecht, J.R., Galan, J., and Birge, R.R. (2003). Protein flexibility and conformational state: a comparison of collective vibrational modes of wild-type and D96N bacteriorhodopsin. Biophys J 85, 1269–1277.
Wilmink, G.J., and Grundt, J.E. (2011). Invited review article: current state of research on biological effects of terahertz radiation. J Infrared Millm W 32, 1074–1122.
Xie, L., Yao, Y. and Ying, Y. (2014). The application of terahertz spectroscopy to protein detection. Appl Spectrosc Rev 49, 448–461.
Zhang, C. (2015). The first artificial Mn4Ca-cluster mimicking the oxygen- evolving center in photosystem II. Sci China Life Sci 58, 816–817.
Zhang, W., Nie, J., and Tu, S. (2015). Study on identification methods in the detection of transgenic material based on terahertz time domain spectroscopy. Opt Quant Electron 47, 3533–3543.
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This work was supported by the Shandong Provincial Natural Science Foundation, China (ZR2012CM020), and the National Training Programs of Innovation and Entrepreneurship for Undergraduates (201410452012).
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Qu, Y., Zhang, S., Lian, Y. et al. Function of terahertz spectra in monitoring the decomposing process of biological macromolecules and in investigating the causes of photoinhibition. Sci. China Life Sci. 60, 307–312 (2017). https://doi.org/10.1007/s11427-016-0057-9
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DOI: https://doi.org/10.1007/s11427-016-0057-9