Abstract
Bifunctional RNAs that possess both protein-coding and noncoding functional properties were less explored and poorly understood. Here we systematically explored the characteristics and functions of such human bifunctional RNAs by integrating tandem mass spectrometry and RNA-seq data. We first constructed a pipeline to identify and annotate bifunctional RNAs, leading to the characterization of 132 high-confidence bifunctional RNAs. Our analyses indicate that bifunctional RNAs may be involved in human embryonic development and can be functional in diverse tissues. Moreover, bifunctional RNAs could interact with multiple miRNAs and RNA-binding proteins to exert their corresponding roles. Bifunctional RNAs may also function as competing endogenous RNAs to regulate the expression of many genes by competing for common targeting miRNAs. Finally, somatic mutations of diverse carcinomas may generate harmful effect on corresponding bifunctional RNAs. Collectively, our study not only provides the pipeline for identifying and annotating bifunctional RNAs but also reveals their important gene-regulatory functions.
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Matched peptides for 132 bifunctional RNAs. Some bifunctional RNAs may encode the same protein, thus they were matched with the same peptide.
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Chen, G., Yang, J., Chen, J. et al. Identifying and annotating human bifunctional RNAs reveals their versatile functions. Sci. China Life Sci. 59, 981–992 (2016). https://doi.org/10.1007/s11427-016-0054-1
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DOI: https://doi.org/10.1007/s11427-016-0054-1