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Science China Life Sciences

, Volume 60, Issue 4, pp 397–403 | Cite as

Novel hemostatic agents based on gelatin-microbial transglutaminase mix

  • Fang Lv
  • Xiaonan Cong
  • Wenshu Tang
  • Yiming Han
  • Yu Tang
  • Yongrui Liu
  • Liqiang Su
  • Mingyao Liu
  • Mingfei JinEmail author
  • Zhengfang YiEmail author
Research Paper

Abstract

Hemostasis is a major challenge in surgical procedures and traumas. Conventional hemostatic methods have limited efficacy and may cause additional tissue damage. In this study, we designed a novel hemostatic agent based on the in situ gel formation of gelatin cross-linked by a novel microbial transglutaminase (mTGase), in which the amino acid sequences differed from commercial mTGases. The new hemostatic agent showed the same biochemical crosslinking chemistry as the final stages of the blood coagulation cascade while using gelatin as a “structural” protein (rather than fibrin) and a calcium-independent mTGase as the crosslinking catalyst (rather than factor XIIIa). In rat liver hemostasis models, the hemostatic agent not only showed a similar hemostatic effect as that of SURGIFLO® (positive control), but also stronger adhesion strength and elasticity than SURGIFLO®. Therefore, this biomimetic gelatin-mTGase mix hemostatic is a novel and effective surgical sealant.

Keywords

microbial transglutaminase gelatin crosslinking biomimetic hemostatic 

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Notes

Acknowledgements

This work was supported by the Major State Basic Research Development Program of China (2015CB910400, 2012CB910400), and National Natural Science Foundation of China (81472788, 81272463, 81330049).

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Copyright information

© Science in China Press and Springer-Verlag GmbH 2017

Authors and Affiliations

  • Fang Lv
    • 1
  • Xiaonan Cong
    • 1
  • Wenshu Tang
    • 1
  • Yiming Han
    • 1
  • Yu Tang
    • 1
  • Yongrui Liu
    • 1
  • Liqiang Su
    • 1
  • Mingyao Liu
    • 1
  • Mingfei Jin
    • 1
    Email author
  • Zhengfang Yi
    • 1
    Email author
  1. 1.Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life SciencesEast China Normal UniversityShanghaiChina

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