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Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014

Science China Life Sciences volume 57, pages 1149–1155 (2014)Cite this article

Abstract

Dengue has been well recognized as a global public health threat, but only sporadic epidemics and imported cases were reported in recent decades in China. Since July 2014, an unexpected large dengue outbreak has occurred in Guangdong province, China, resulting in more than 40000 patients including six deaths. To clarify and characterize the causative agent of this outbreak, the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing (HTS). Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2 (DENV-2). A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells. The complete genome of GZ05/2014 was determined and deposited in GenBank under the access number KP012546. Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade. No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014. The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong, China. These results help clarify the origin of this epidemic and predict the future status of dengue in China.

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Affiliations

  1. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China

    Hui Zhao, Tao Jiang, XiaoFeng Li, Hang Fan, Yu Zhang, Qing Ye, YiGang Tong, WuChun Cao & ChengFeng Qin

  2. Guangzhou 8th People’s Hospital, Guangzhou Medical University, Guangzhou, 510060, China

    LingZhai Zhao, WenXin Hong, Qin Zhu & FuChun Zhang

Authors
  1. Hui Zhao
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  2. LingZhai Zhao
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  3. Tao Jiang
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  4. XiaoFeng Li
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  5. Hang Fan
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  6. WenXin Hong
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  7. Yu Zhang
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  8. Qin Zhu
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  9. Qing Ye
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  10. YiGang Tong
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  11. WuChun Cao
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  12. FuChun Zhang
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  13. ChengFeng Qin
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Corresponding authors

Correspondence to FuChun Zhang or ChengFeng Qin.

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Zhao, H., Zhao, L., Jiang, T. et al. Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014. Sci. China Life Sci. 57, 1149–1155 (2014). https://doi.org/10.1007/s11427-014-4782-3

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  • DOI: https://doi.org/10.1007/s11427-014-4782-3

Keywords

  • dengue virus serotype 2
  • virus isolation
  • phylogenetic analysis
  • envelope gene