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CRISPR-Cas9 knockout screening for functional genomics


  1. Gaj T, Gersbach CA, Barbas CF. ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol, 2013, 31: 397–405

    PubMed  CAS  PubMed Central  Article  Google Scholar 

  2. Zhou Y, Zhu S, Cai C, Yuan PF, Li CM, Huang YY, Wei WS. High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature, 2014, 509: 487–491

    PubMed  CAS  Article  Google Scholar 

  3. Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K. Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Nat Biotechnol, 2013, 32: 267–273

    PubMed  Article  Google Scholar 

  4. Wang T, Wei JJ, Sabatini DM, Lander ES. Genetic screens in human cells using the CRISPR-Cas9 system. Science, 2013, 343: 80–84

    PubMed  PubMed Central  Article  Google Scholar 

  5. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 2013, 343: 84–87

    PubMed  PubMed Central  Article  Google Scholar 

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Correspondence to GuangShuo Ou.

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Shen, Z., Ou, G. CRISPR-Cas9 knockout screening for functional genomics. Sci. China Life Sci. 57, 733–734 (2014).

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