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Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella

Science China Life Sciences volume 56, pages 59–65 (2013)Cite this article

Abstract

Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus’s structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse naïve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5–7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.

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Affiliations

  1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China

    CongLin Chen, LanJie Liao, ShaoXiang Luo, ZhouQuan Li, XiaoHua Zhang, YaPing Wang, QionLin Guo & HePing Dai

  2. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    XiaoYun Sun & Qin Fang

Authors
  1. CongLin Chen
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  2. XiaoYun Sun
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  3. LanJie Liao
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  4. ShaoXiang Luo
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  5. ZhouQuan Li
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  6. XiaoHua Zhang
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  7. YaPing Wang
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  8. QionLin Guo
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  9. Qin Fang
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  10. HePing Dai
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Correspondence to Qin Fang or HePing Dai.

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Chen, C., Sun, X., Liao, L. et al. Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella. Sci. China Life Sci. 56, 59–65 (2013). https://doi.org/10.1007/s11427-012-4425-5

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  • DOI: https://doi.org/10.1007/s11427-012-4425-5

Keywords

  • grass carp
  • grass carp reovirus (GCRV)
  • IgM
  • single-chain variable fragment (scFv)
  • antigenicity