Abstract
Nitrogen (N) is an essential macronutrient and an important factor limiting agricultural productivity. N deficient or excess conditions often occur during the cotton growth season and incorrect N application may affect cotton fiber yield and quality. Here, the influence of N stress on the cotton fiber proteome was investigated by two-dimensional gel electrophoresis and mass spectrometry. The results indicated that N application rate affects nitrogen accumulation in fiber cells and fiber length. The proteins differentially expressed during N stress were mainly related to plant carbohydrate metabolism, cell wall component synthesis and transportation, protein/amino acid metabolism, antioxidation and hormone metabolism. The most abundant proteins were C metabolism-related. Ten days post anthesis is a critical time for fiber cells to perceive environmental stress and most proteins were suppressed in both N deficient and N excess conditions at this sampling stage. However, several N metabolism proteins were increased to enhance N stress tolerance. Excess N may suppress carbohydrate/energy metabolism in early fiber development much like N deficiency. These results have identified some interesting proteins that can be further analyzed to elucidate the molecular mechanisms of N tolerance.
Article PDF
Similar content being viewed by others
References
Ramey Jr H H. Stress influences on fiber development. In: Mauney J R, Stewart J M, eds. Cotton Physiology. Memphis: The Cotton Foundation, 1986. 351–359
Reddy K R, Koti S, Davidonis G H, et al. Interactive effects of carbon dioxide and nitrogen nutrition on cotton growth, development, yield, and fiber quality. Agron J, 2004, 96: 1148–1157
Read J J, Reddy K R, Jenkins J N. Yield and fiber quality of upland cotton as influenced by nitrogen and potassium nutrition. Eur J Agron, 2006, 24: 282–290
Rochester I, Peoples M, Constable G. Estimation of the N fertiliser requirement of cotton grown after legume crops. Field Crop Res, 2001, 70: 43–53
Wang R, Tischner R, Gutierrez R A, et al. Genomic analysis of the nitrate response using a nitrate reductase-null mutant of Arabidopsis. Plant Physiol, 2004, 136: 2512–2522
Wang R, Okamoto M, Xing X, et al. Microarray analysis of the nitrate response in Arabidopsis roots and shoots reveals over 1000 rapidly responding genes and new linkages to glucose, trehalose-6-phosphate, iron, and sulfate metabolism. Plant Physiol, 2003, 132: 556–567
Scheible W R, Morcuende R, Czechowski T, et al. Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen. Plant Physiol, 2004, 136: 2483–2499
Flaete N, Hollung K, Ruud L, et al. Combined nitrogen and sulphur fertilisation and its effect on wheat quality and protein composition measured by SE-FPLC and proteomics. J Cereal Sci, 2005, 41: 357–369
Bahrman N, Gouy A, Devienne-Barret F, et al. Differential change in root protein patterns of two wheat varieties under high and low nitrogen nutrition levels. Plant Sci, 2005, 168: 81–87
Bahrman N, Le Gouis J, Negroni L, et al. Differential protein expression assessed by two-dimensional gel electrophoresis for two wheat varieties grown at four nitrogen levels. Proteomics, 2004, 4: 709–719
Tran N P, Park J K, Lee C G. Proteomics analysis of proteins in green alga Haematococcus lacustris (Chlorophyceae) expressed under combined stress of nitrogen starvation and high irradiance. Enzyme Microb Technol, 2009, 45: 241–246
Konishi H, Ishiguro K, Komatsu S. A proteomics approach towards understanding blast fungus infection of rice grown under different levels of nitrogen fertilization. Proteomics, 2001, 1: 1162–1171
Dhugga K S, Waines J G, Leonard R T. Correlated induction of nitrate uptake and membrane polypeptides in corn roots. Plant Physiol, 1988, 87: 120–125
Zhao D H, Li J L, Qi J G. Identification of red and NIR spectral regions and vegetative indices for discrimination of cotton nitrogen stress and growth stage. Comput Electron Agric, 2005, 48: 155–169
Graves D A, Stewart J M D. Analysis of the protein constituency of developing cotton fibres. J Exp Bot, 1988, 39: 59–69
Turley R, Ferguson D. Changes of ovule proteins during early fiber development in a normal and a fiberless line of cotton (Gossypium hirsutum L.). J Plant Physiol, 1996, 149: 695–702
Yao Y, Yang Y W, Liu J Y. An efficient protein preparation for proteomic analysis of developing cotton fibers by 2-DE. Electrophoresis, 2006, 27: 4559–4569
Yang Y W, Bian S M, Yao Y, et al. Comparative proteomic analysis provides new insights into the fiber elongating process in cotton. J Proteome Res, 2008, 7: 4623–4637
Feil B, Moser S B, Jampatong S, et al. Mineral composition of the grain of tropical maize varieties as affected by pre-anthesis drought and rate of nitrogen fertilization. Crop Sci, 2005, 45: 516–523
Gipson J R, Ray L L. Fibre elongation rates in five varieties of cotton as influenced by night temperature. Crop Sci, 1969, 9: 339–341
Bhushan D, Pandey A, Choudhary M K, et al. Comparative proteomics analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress. Mol Cell Proteomics, 2007, 6: 1868–1884
Hearn A, Constable G. Irrigation for crops in a sub-humid environment VII. Evaluation of irrigation strategies for cotton. Irrig Sci, 1984, 5: 75–94
Singh V, Nagwekar S. Effect of weed control and nitrogen levels on quality characters in cotton. J Indian Soc Cotton Improvement, 1989, 14: 60–64
Anderson C M, Wagner T A, Perret M, et al. WAKs: cell wall-associated kinases linking the cytoplasm to the extracellular matrix. Plant Mol Biol, 2001, 47: 197–206
Chen F, Li Q, Sun L, et al. The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress. DNA Res, 2006, 13: 53–63
Cotelle V, Meek S E M, Provan F, et al. 14-3-3s regulate global cleavage of their diverse binding partners in sugar-starved Arabidopsis cells. EMBO J, 2000, 19: 2869–2876
Gonda T J. The c-Myb oncoprotein. Int J Biochem Cell B, 1998, 30: 547–551
Todd C D, Zeng P, Huete A M R, et al. Transcripts of MYB-like genes respond to phosphorous and nitrogen deprivation in Arabidopsis. Planta, 2004, 219: 1003–1009
Bowler C, Chua N H. Emerging themes of plant signal transduction. Plant Cell, 1994, 6: 1529–1541
Means A R, Dedman J R. Calmodulin—an intracellular calcium receptor. Nature, 1980, 285: 73–77
Miyashita Y, Dolferus R, Ismond K P, et al. Alanine aminotransferase catalyses the breakdown of alanine after hypoxia in Arabidopsis thaliana. Plant J, 2007, 49: 1108–1121
Son D, Jo J, Sugiyama T. Purification and characterization of alanine aminotransferase from Panicum miliaceum leaves. Arch Biochem Biophys, 1991, 289: 262–266
Muench D G, Christopher M E, Good A G. Cloning and expression of a hypoxic and nitrogen inducible maize alanine aminotransferase gene. Physiol Plant, 1998, 103: 503–512
Fuentes S I, Allen D J, Ortiz-Lopez A, et al. Over-expression of cytosolic glutamine synthetase increases photosynthesis and growth at low nitrogen concentrations. J Exp Bot, 2001, 52: 1071–1081
Chao W S, Gu Y Q, Pautot V, et al. Leucine aminopeptidase RNAs, proteins, and activities increase in response to water deficit, salinity, and the wound signals systemin, methyl jasmonate, and abscisic acid. Plant Physiol, 1999, 120: 979–992
Claussen M, Lüthe H, Blatt M, et al. Auxin-induced growth and its linkage to potassium channels. Planta, 1997, 201: 227–234
Xiao Y H, Li D M, Yin M H, et al. Gibberellin 20-oxidase promotes initiation and elongation of cotton fibers by regulating gibberellin synthesis. J Plant Physiol, 2010, 167: 829–837
Author information
Authors and Affiliations
Corresponding author
Additional information
This article is published with open access at Springerlink.com
Contributed equally to this work
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Wang, Y., Zheng, M., Gao, X. et al. Protein differential expression in the elongating cotton (Gossypiumhirsutum L.) fiber under nitrogen stress. Sci. China Life Sci. 55, 984–992 (2012). https://doi.org/10.1007/s11427-012-4390-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11427-012-4390-z