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Qualitative and quantitative analyses of influenza virus receptors in trachea and lung tissues of humans, mice, chickens and ducks

Science China Life Sciences volume 55, pages 612–617 (2012)Cite this article

Abstract

To accurately determine the expression and distribution patterns of two influenza virus receptors (SAα2,3-gal and SAα2,6-gal) in trachea and lung tissues of humans, mice, chickens and ducks, we analyzed lectin immunofluorescence stainings of various tissue sections qualitatively and quantitatively. Results from the qualitative analysis showed that both influenza virus receptors were expressed in lung tissues of humans, mice, chickens and ducks as well as trachea tissues of mice and ducks. However, SAα2,6-gal receptor was expressed only in the human trachea tissue and SAα2,3-gal receptor was expressed only in the chicken trachea tissue. Results from the quantitative analysis demonstrated that both receptors were expressed in trachea tissues of human and mouse, as well as in lung tissues of humans, chickens and ducks. Meanwhile, our results also showed that the expression and distribution of influenza virus receptors in the same tissue were not always uniform, indicating that their distribution and expression in various tissues are not simply the distinction between the presence or absence of receptors, but rather the difference in the amount of expressed receptors.

References

  1. Korteweg C, Gu J. Pathology, molecular biology, and pathogenesis of avian influenza A (H5N1) infection in humans. Am J Pathol, 2008, 172: 1155–1170

    PubMed  CAS  PubMed Central  Article  Google Scholar 

  2. Tollis M, Di Trani L. Recent developments in avian influenza research: epidemiology and immunoprophylaxis. Vet J, 2002, 164: 202–215

    PubMed  CAS  Article  Google Scholar 

  3. Ge S, Wang Z. An overview of influenza A virus receptors. Crit Rev Microbiol, 2011, 37: 157–165

    PubMed  CAS  Article  Google Scholar 

  4. Zhang H. Tissue and host tropism of influenza viruses: Importance of quantitative analysis. Sci China Ser C-Life Sci, 2009, 52: 1101–1110

    Article  Google Scholar 

  5. Wang W C, Cummings R D. The immobilized leukoagglutinin from the seeds of Maackia amurensis binds with high affinity to complex-type Asn-linked oligosaccharides containing terminal sialic acid-linked alpha-2,3 to penultimate galactose residues. J Biol Chem, 1988, 263: 4576–4585

    PubMed  CAS  Google Scholar 

  6. Mach L, Scherf W, Ammann M, et al. Purification and partial characterization of a novel lectin from elder (Sambucus nigra L.) fruit. Biochem J, 1991, 278: 667–671

    PubMed  CAS  PubMed Central  Article  Google Scholar 

  7. Zhou G Y. Histopathological Technology. Beijing: Peking University Medical Press, 2006. 1–72

    Google Scholar 

  8. Centers for Disease Control and Prevention (CDC). Isolation of avian influenza A (H5N1) viruses from humans-Hong Kong, May–December 1997. MMWR Morb Mortal Wkly Rep, 1997, 46: 1204–1207

    Google Scholar 

  9. Ning Z Y, Luo M Y, Qi W B, et al. Detection of expression of influenza virus receptors in tissues of BALB/c mice by histochemistry. Vet Res Commun, 2009, 33: 895–903

    PubMed  Article  Google Scholar 

  10. Ning Z Y, Liu X M, Luo M Y. Distribution of influenza virus receptors in trachea and lung of three animal detected by histochemistry. Chin J Animal Health Inspect, 2009, 4: 44–47

    Google Scholar 

  11. Kuchipudi S V, Nelli R, White G A, et al. Differences in influenza virus receptors in chickens and ducks: Implications for interspecies transmission. J Mol Genet Med, 2009, 3: 143–151

    PubMed  CAS  PubMed Central  Article  Google Scholar 

  12. Gambaryan A, Webster R, Matrosovich M. Differences between influenza virus receptors on target cells of duck and chicken. Arch Virol, 2002, 147: 1197–1208

    PubMed  CAS  Article  Google Scholar 

  13. Ibricevic A, Pekosz A, Walter M J, et al. Influenza virus receptor specificity and cell tropism in mouse and human airway epithelial cells. J Virol, 2006, 80: 7469–7480

    PubMed  CAS  PubMed Central  Article  Google Scholar 

  14. Dai W T, Han X, Wang H L. Analysis difference of HearNPV duplication in log phase and platform. Chin J Virol, 2007, 23: 399–406

    CAS  Google Scholar 

  15. Yang B, Zhou Y C, Li S T. Effects of acute denervation on ratio change of nicotinic acetylcholine receptor subtypes in the rat gastrocnemius muscle. J Pract Med, 2008, 24: 4012–4014

    CAS  Google Scholar 

  16. Zhang X H, Chen Z P. The application of somatostatin analogues in the patients with GH—secreting pituitary adenoma. Chin J Neurooncol, 2008, 6: 69–71

    Google Scholar 

  17. Deng Y L, Li C Y. Advance in the study of the idiopathic hypercalciuria. Int J Urol Nephrol, 2007, 27: 371–374

    Google Scholar 

  18. Vudattu N K, Kuhlmann-Berenzon S, Khademi M, et al. Increased numbers of IL-7 receptor molecules on CD4+CD25-CD107a+ T-cells in patients with autoimmune diseases affecting the central nervous system. PLoS ONE, 2009, 4: e6534

    PubMed  PubMed Central  Article  Google Scholar 

  19. Tanaka J, Matsuzaki M, Tarusawa E, et al. Number and density of AMPA receptors in single synapses in immature cerebellum. J Neurosci, 2005, 25: 799–807

    PubMed  CAS  Article  Google Scholar 

  20. Desmetz C, Lin Y L, Mettling C, et al. The strength of the chemotactic response to a CCR5 binding chemokine is determined by the level of cell surface CCR5 density. Immunology, 2006, 119: 551–5611

    PubMed  CAS  PubMed Central  Article  Google Scholar 

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Affiliations

  1. Laboratory of Respiratory Medicine, Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi, 563003, China

    BaoLin Wang, Bo Liu, Ling Chen, JianYong Zhang & Hong Zhang

  2. National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China

    HongXuan He & Hong Zhang

  3. Z-BioMed, Inc., Rockville, MD, 20855, USA

    Hong Zhang

Authors
  1. BaoLin Wang
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  2. Bo Liu
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  3. Ling Chen
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  4. JianYong Zhang
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  5. HongXuan He
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  6. Hong Zhang
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Corresponding authors

Correspondence to Ling Chen or Hong Zhang.

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Open Access This article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wang, B., Liu, B., Chen, L. et al. Qualitative and quantitative analyses of influenza virus receptors in trachea and lung tissues of humans, mice, chickens and ducks. Sci. China Life Sci. 55, 612–617 (2012). https://doi.org/10.1007/s11427-012-4341-8

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  • DOI: https://doi.org/10.1007/s11427-012-4341-8

Keywords

  • influenza virus
  • sialic acid receptors
  • trachea
  • lung
  • lectin
  • immunofluorescence
  • quantitative analysis
  • qualitative analysis