Abstract
Non-structural protein 1 (NS1) of the influenza virus plays a crucial role in modulating the host immune response and facilitating virus replication. The formation of a homodimer or an oligomer is necessary for NS1 to exert its function efficiently. In the present study, the NS1 protein from the A/Shantou/602/06(H3N2) virus (herein abbreviated as NS32) was found to interact with NS1 from A/Shantou/169/06(H1N1), A/Chicken/Guangdong/1/05(H5N1) and A/Quail/Hong Kong/G1/97(H9N2) (abbreviated as NS11, NS51 and NS92, respectively) viruses, although NS32 shares 17.4%–20.9% sequence diversity with NS11, NS51 and NS92. This indicates that the heterologous interactions between NS1 proteins from different influenza A virus subtypes/ strains may be a common event during co-infection.
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Fouchier R A, Munster V, Wallensten A, et al. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls. J Virol, 2005, 79: 2814–2822
Hale B G, Randall R E, Ortin J, et al. The multifunctional NS1 protein of influenza A viruses. J Gen Virol, 2008, 89: 2359–2376
Chien C Y, Tejero R, Huang Y, et al. A novel RNA-binding motif in influenza A virus non-structural protein 1. Nat Struct Biol, 1997, 4: 891–895
Chien C Y, Xu Y, Xiao R, et al. Biophysical characterization of the complex between double-stranded RNA and the N-terminal domain of the NS1 protein from influenza A virus: evidence for a novel RNA-binding mode. Biochemistry, 2004, 43: 1950–1962
Cheng A, Wong S M, Yuan Y A. Structural basis for dsRNA recognition by NS1 protein of influenza A virus. Cell Res, 2009, 19: 187–195
Liu J, Lynch P A, Chien C Y, et al. Crystal structure of the unique RNA-binding domain of the influenza virus NS1 protein. Nat Struct Biol, 1997, 4: 896–899
Hale B G, Barclay W S, Randall R E, et al. Structure of an avian influenza A virus NS1 protein effector domain. Virology, 2008, 378: 1–5
Bornholdt Z A, Prasad B V. X-ray structure of influenza virus NS1 effector domain. Nat Struct Mol Biol, 2006, 13: 559–560
Wang W, Riedel K, Lynch P, et al. RNA binding by the novel helical domain of the influenza virus NS1 protein requires its dimer structure and a small number of specific basic amino acids. RNA, 1999, 5: 195–205
Wang X, Basler C F, Williams B R, et al. Functional replacement of the carboxy-terminal two-thirds of the influenza A virus NS1 protein with short heterologous dimerization domains. J Virol, 2002, 76: 12951–12962
Li K S, Guan Y, Wang J, et al. Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia. Nature, 2004, 430: 209–213
Guan Y, Poon L L, Cheung C Y, et al. H5N1 influenza: a protean pandemic threat. Proc Natl Acad Sci USA, 2004, 101: 8156–8161
Ludwig S, Schultz U, Mandler J, et al. Phylogenetic relationship of the nonstructural (NS) genes of influenza A viruses. Virology, 1991, 183: 566–577
Dundon W G, Capua I. A Closer Look at the NS1 of Influenza Virus. Viruses, 2009, 1: 1057–1072
Scholtissek C, Rohde W, Von Hoyningen V, et al. On the origin of the human influenza virus subtypes H2N2 and H3N2. Virology, 1978, 87: 13–20
Kawaoka Y, Krauss S, Webster R G. Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics. J Virol, 1989, 63: 4603–4608
Schafer J R, Kawaoka Y, Bean W J, et al. Origin of the pandemic 1957 H2 influenza A virus and the persistence of its possible progenitors in the avian reservoir. Virology, 1993, 194: 781–788
Smith G J, Vijaykrishna D, Bahl J, et al. Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic. Nature, 2009, 459: 1122–1125
Falchi A, Arena C, Andreoletti L, et al. Dual infections by influenza A/H3N2 and B viruses and by influenza A/H3N2 and A/H1N1 viruses during winter 2007, Corsica Island, France. J Clin Virol, 2008, 41: 148–151
Peacey M, Hall R J, Sonnberg S, et al. Pandemic (H1N1) 2009 and seasonal influenza A (H1N1) co-infection, New Zealand, 2009. Emerg Infect Dis, 2010, 16: 1618–1620
Ghedin E, Fitch A, Boyne A, et al. Mixed infection and the genesis of influenza virus diversity. J Virol, 2009, 83: 8832–8841
Dugan V G, Chen R, Spiro D J, et al. The evolutionary genetics and emergence of avian influenza viruses in wild birds. PLoS Pathog, 2008, 4: e1000076
Sharp G B, Kawaoka Y, Jones D J, et al. Coinfection of wild ducks by influenza A viruses: distribution patterns and biological significance. J Virol, 1997, 71: 6128–6135
Hayman A, Comely S, Lackenby A, et al. Variation in the ability of human influenza A viruses to induce and inhibit the IFN-beta pathway. Virology, 2006, 347: 52–64
Melen K, Kinnunen L, Fagerlund R, et al. Nuclear and nucleolar targeting of influenza A virus NS1 protein: striking differences between different virus subtypes. J Virol, 2007, 81: 5995–6006
Murayama R, Harada Y, Shibata T, et al. Influenza A virus non-structural protein 1 (NS1) interacts with cellular multifunctional protein nucleolin during infection. Biochem Biophys Res Commun, 2007, 362: 880–885
Zhang D G, Li W Z, Wang G F, et al. PL Domain at the carboxyl terminus of influenza A virus NS1 protein influences the nuclear localization of NS1 (in Chinese). Prog Biochem Biophys, 2010, 37: 975–982
Kim M J, Latham A G, Krug R M. Human influenza viruses activate an interferon-independent transcription of cellular antiviral genes: outcome with influenza A virus is unique. Proc Natl Acad Sci USA, 2002, 99: 10096–10101
Li W Z, Wang G F, Zeng J, et al. Differential transcription-activating capability of NS1 proteins from different influenza virus subtypes expressed in yeast. Sci China Ser C-Life Sci, 2009, 52: 545–550
Nemeroff M E, Qian X Y, Krug R M. The influenza virus NS1 protein forms multimers in vitro and in vivo. Virology, 1995, 212: 422–428
Bornholdt Z A, Prasad B V. X-ray structure of NS1 from a highly pathogenic H5N1 influenza virus. Nature, 2008, 456: 985–988
Kochs G, Garcia-Sastre A, Martinez-Sobrido L. Multiple anti-interferon actions of the influenza A virus NS1 protein. J Virol, 2007, 81: 7011–7021
de Vries W, Haasnoot J, Fouchier R, et al. Differential RNA silencing suppression activity of NS1 proteins from different influenza A virus strains. J Gen Virol, 2009, 90: 1916–1922
Li W Z, Wang G F, Zhang H, et al. Inability of NS1 protein from an H5N1 influenza virus to activate PI3K/Akt signaling pathway correlates to the enhanced virus replication upon PI3K inhibition. Vet Res, 2012, 43: 36
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Li, W., Zhang, H., Wang, G. et al. Heterologous interactions between NS1 proteins from different influenza A virus subtypes/strains. Sci. China Life Sci. 55, 507–515 (2012). https://doi.org/10.1007/s11427-012-4335-6
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DOI: https://doi.org/10.1007/s11427-012-4335-6