Abstract
Carbon nitride (CN x ) and diamond-like carbon (DLC) coatings were prepared by dc magnetron sputtering at room temperature. Different partial pressures of N2 were used to synthesize CN x to evaluate the relationship between the atomic percentage of nitrogen and hemocompatibility. Auger electron spectroscopy and atomic force microscopy indicated atomic percentages of N of 0.12 and 0.22 and that the CN x coatings were smooth. An in vitro study of the hemocompatibility of the coatings revealed that both CN x coatings had better anticoagulant properties and lower platelet adhesion than DLC. Compared with CN0.12, the CN0.22 coating showed longer dynamic clotting time (about 42 min), static clotting time (23.6 min) and recalcification time (45.6 s), as well as lower platelet adhesion (102 cells μm−2), aggregation, and activation. The presence of nitrogen in the CN x coatings induced their enhanced hemocompatibility compared with DLC.
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Ruckenstein E, Gourisankar S V. Preparation and characterization of thin film surface coatings for biological environments. Biomaterials, 1986, 7: 403–422
Jelinek M, Smetana K, Kocoureka T, et al. Biocompatibility and sp3/sp2 ratio of laser created DLC films. Mater Sci Eng B, 2010, 169: 89–93
Yang P, Huang N, Leng Y X, et al. Activation of platelets adhered on amorphous hydrogenated carbon (a-C:H) films synthesized by plasma immersion ion implantation-deposition (PIII-D). Biomaterials, 2003, 24: 2821–2829
Salgueiredo E, Vila M, Silva M A, et al. Biocompatibility evaluation of DLC-coated Si3N4 substrates for biomedical applications. Dia Rel Mater, 2008, 17: 878–881
Bharathy P V, Nataraj D, Chu P K, et al. Effect of titanium incorporation on the structural, mechanical and biocompatible properties of DLC thin films prepared by reactive-biased target ion beam deposition method. Appl Surf Sci, 2010, 257: 143–150
Pandiyaraj K N, Selvarajan V, Heeg J, et al. Influence of bias voltage on diamond like carbon (DLC) film deposited on polyethylene terephthalate (PET) film surfaces using PECVD and its blood compatibility. Dia Rel Mater, 2010, 19: 1085–1092
Dearnaley G, Arps J H. Biomedical applications of diamond-like carbon (DLC) coatings: A review. Surf Coat Technol, 2005, 200: 2518–2524
Maa W J, Ruysa A J, Masonb R S, et al. Use of diamond-like carbon with tungsten (W-DLC) films as biocompatible material. Biomaterials, 2007, 28: 1620–1628
Lousinian S, Logothetidis S, Laskarakis A, et al. Haemocompatibility of amorphous hydrogenated carbon thin films, optical properties and adsorption mechanisms of blood plasma proteins. Biomol Eng, 2007, 24: 107–112
Uzumaki E T, Lambert C S, Santos A R, et al. Surface properties and cell behaviour of diamond-like carbon coatings produced by plasma immersion. Thin Solid Films, 2006, 515: 293–300
Allen M, Myer B, Rushton N. In vitro and in vivo investigations into the biocompatibility of diamond-like carbon (DLC) coatings for orthopedic applications. J Biomed Mater Res, 2001, 58: 319–328
Cui F Z, Qing X L, Li D J, et al. Biomedical investigations on CNx coating. Surf Coat Technol, 2005, 200: 1009–1013
Jones M I, McColl I R, Grant D M, et al. Structure and properties of annealed amorphous hydrogenated carbon (a-C:H) films for biomedical applications. Dia Rel Mater, 1999, 8: 457–462
Bendavid A, Martin P J, Comte C, et al. The mechanical and biocompatibility properties of DLC-Si films prepared by pulsed DC plasma activated chemical vapor deposition. Dia Rel Mater, 2007, 16: 1616–1622
Marton M, Zdravecka E, Vojs M, et al. Study of adhesion of carbon nitride thin films on medical alloy substrates. Vacuum, 2010, 84: 65–67
Rossi F, Andre B, Van Veen A, et al. Physical properties of nitrogenated amorphous carbon films produced by ion-beam-assisted deposition. Thin Solid Films, 1994, 253: 85–89
Yu L J, Wang X, Wang X H, et al. Haemocompatibility of tetrahedral amorphous carbon films. Surf Coat Technol, 2000, 128–129: 484–488
Zheng C L, Cui F Z, Meng B, et al. Hemocompatibility of C-N films fabricated by ion beam assisted deposition. Surf Coat Technol, 2005, 193: 361–365
Li D J, Guruz M U, Bhatia C S, et al. Ultrathin CNx overcoats for 1 Tb/in.2 hard disk drive systems. Appl Phys Lett, 2002, 81: 1113–1115
Milojka G, Gerhard S, Wolfgang G, et al. A comparison of different methods to calculate the surface free energy of wood using contact angle measurements. Colloids Surf A Physicochem Eng Asp, 2001, 181: 279–287
Dynes P J, Kaelble D H. Surface energy analysis of carbon fibers and films. J Adhes, 1974, 6: 195–199
Laura J S, Jennifer L W, Antonios G M. Platelet adhesion on a bioresorbable poly(propylene fumarate-co-ethylene glycol) copolymer. Biomaterials, 1999, 20: 683–690
Ivarsson B, Lundstrom I. Physical characterization of protein adsorption on metal and metaloxide surfaces. CRC Crit Rev Biocompat, 1986, 2: 1–96
Baier R E. Conditioning surfaces to suit the biomedical environment. J Biomech Eng, 1982, 104: 257–260
Baurschmidt P, Schaldach M. Alloplastic materials for heart-valve prostheses. Med Biol Eng Comput, 1980, 18: 496–502
Zhang L, Chen M, Li Z Y, et al. Effect of annealing on structure and haemocompatibility of tetrahedral amorphous hydrogenated carbon films. Mater Lett, 2008, 62: 1040–1043
Titantah J T, Lamoen D. Carbon and nitrogen 1s energy levels in amorphous carbon nitride systems: XPS interpretation using first-principles. Dia Rel Mater, 2007, 16: 581–588
Hultman L, Neidhardt J, Hellgren N, et al. Fullerene-like carbon nitride: A resilient coating material. MRS Bull, 2003, 28: 194–202
Ferrari A C, Kleinsorge B, Morrison N A, et al. Stress reduction and bond stability during thermal annealing of tetrahedral amorphous carbon. J Appl Phys, 1999, 85: 7191–7197
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Zhao, M., Li, D., Zhang, Y. et al. In vitro comparison of the hemocompatibility of diamond-like carbon and carbon nitride coatings with different atomic percentages of N. Sci. China Life Sci. 55, 343–348 (2012). https://doi.org/10.1007/s11427-012-4308-9
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DOI: https://doi.org/10.1007/s11427-012-4308-9