Abstract
hb (hunchback) is a contributing factor in anteroposterior axial patterning of insects. Although the hb function in Locusta migratoria manilensis has been investigated, its expression pattern remains unknown. Here, the mouse polyclonal antibody was produced against Hb fusion protein, and then its expression pattern during oogenesis and embryogenesis of L. migratoria manilensis was examined by immunohistochemical staining. Hb protein was detected in the oocyte nucleus which was positioned centrally within the developing oocyte. The oocyte nucleus gradually moved to the posterior end of the egg along with the oocyte maturing. In freshly laid eggs, Hb formed gradient at the posterior end of the egg, and then hb was expressed as a band in the middle of the blastodisc. As the blastodisc differentiated into the head and trunk, the expression region became wide, which would develop into spatial gnathal and thoracic segments. With abdominal segmentation, the expression domain in the gnathal and thoracic region became faint and eventually faded out, while the Hb expression domain appeared at the posterior growth zone in a discontinuous expression manner. The hb expression pattern of L. migratoria manilensis is greatly similar to that of other locusts, such as Schistocerca americana and another L. migratoria. Compared with other insects, hb expression is conserved in the gnathal and thoracic domains, while divergent in oogenesis and abdomen.
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Davis G K, Patel N H. Short, long, and beyond: molecular and embryological approaches to insect segmentation. Annu Rev Entomol, 2002, 47:669–699, 1:CAS:528:DC%2BD38XnvVWltw%3D%3D, 10.1146/annurev.ento.47.091201.145251, 11729088
Liu P Z, Kaufman T C. hunchback is required for suppression of abdominal identity, and for proper germband growth and segmentation in the intermediate germband insect Oncopeltus fasciatus. Development, 2004, 131:1515–1527, 1:CAS:528:DC%2BD2cXjsFKltbo%3D, 10.1242/dev.01046, 14998925
Sander K, Gutzeit H, Jäckle H. Insect embryogenesis: morphology, physiology, and genetical and molecular aspects. In: Kerkut G A, Gilbert L I, eds. Comprehensive Insect Physiology Biochemistry and Pharmacology, Vol. 1. Oxford: Pergamon Press, 1985. 319–385
Rivera-Pomar R, Jackle H. From gradients to stripes in Drosophila embryogenesis: filling in the gaps. Trends Genet, 1996, 12: 478–483, 1:CAS:528:DyaK28XntVKnsbg%3D, 10.1016/0168-9525(96)10044-5, 8973159
St Johnston D, Nüsslein-Volhard C. The origin of pattern and polarity in the Drosophila embryo. Cell, 1992, 68:201–219, 1:CAS:528:DyaK38XhsVynurg%3D, 10.1016/0092-8674(92)90466-P, 1733499
He Z B, Cao Y Q, Wang Z K, et al. Role of hunchback in segment patterning of Locusta migratoria manilensis revealed by parental RNAi. Dev Growth Differ, 2006, 48:439–445, 1:CAS:528:DC%2BD28XhtFSjs7nK, 10.1111/j.1440-169X.2006.00881.x, 16961591
Patel N H, Hayward D C, Lall S, et al. Grasshopper hunchback expression reveals conserved and novel aspects of axis formation and segmentation. Development, 2001, 128:3459–3472, 1:CAS:528:DC%2BD3MXnslCqu7o%3D, 11566852
Tautz D, Lehmann R, Schnürch H, et al. Finger protein of novel structure encoded by hunchback, a second member of the gap class of Drosophila segmentation genes. Nature, 1987, 327:383–389, 1:CAS:528:DyaL2sXlvFajtr4%3D, 10.1038/327383a0
Simpson-Brose M, Treisman J, Desplan C. Synergy between the hunchback and bicoid morphogens is required for anterior patterning in Drosophila. Cell, 1994, 78:855–865, 1:STN:280:DyaK2czntlyhsw%3D%3D, 10.1016/S0092-8674(94)90622-X, 8087852
Casares F, Sánchez-Herrero E. Regulation of the infraabdominal regions of the bithorax complex of Drosophila by gap genes. Development, 1995, 121:1855–1866, 1:CAS:528:DyaK2MXmsVamtro%3D, 7600999
Tautz D, Sommer R. Evolution of segmentation genes in insects. Trends Genet, 1995, 11:23–27, 1:CAS:528:DyaK2MXjtFGjtbg%3D, 10.1016/S0168-9525(00)88982-9, 7900191
Wu X, Vasisht V, Kosman D, et al. Thoracic patterning by the Drosophila gap gene, hunchback. Dev Biol, 2001, 237:79–92, 1:CAS:528:DC%2BD3MXmtFGgsbo%3D, 10.1006/dbio.2001.0355, 11518507
Clyde D E, Corado M S, Wu X, et al. A self-organizing system of repressor gradients establishes segmental complexity in Drosophila. Nature, 2003, 426:849–853, 1:CAS:528:DC%2BD3sXpvVGmtLw%3D, 10.1038/nature02189, 14685241
Lehmann R, Nüsslein-Volhard C. hunchback, a gene required for segmentation of anterior and posterior regions of the Drosophila embryo. Dev Biol, 1987, 119:402–417, 1:STN:280:DyaL2s7gs1entw%3D%3D, 10.1016/0012-1606(87)90045-5, 3803711
Schröder R. The genes orthodenticle and hunchback substitute for bicoid in the beetle Tribolium. Nature, 2003, 422:621–625, 10.1038/nature01536, 12687002
Pultz M A, Pitt J N, Alto N M. Extensive zygotic control of the anteroposterior axis in the wasp Nasonia vitripennis. Development, 1999, 126:701–710, 1:CAS:528:DyaK1MXhvFanu7w%3D, 9895318
Pultz M A, Westendorf L, Gale S D, et al. A major role for zygotic hunchback in patterning the Nasonia embryo. Development, 2005, 132:3705–3715, 1:CAS:528:DC%2BD2MXhtVaqurbP, 10.1242/dev.01939, 16077090
Mito T, Sarashina I, Zhang H, et al. Non-canonical functions of hunchback in segment patterning of the intermediate germ cricket Gryllus bimaculatus. Development, 2005, 132:2069–2079, 1:CAS:528:DC%2BD2MXltFagsb0%3D, 10.1242/dev.01784, 15788457
Dong Y, Friedric H M. Nymphal RNAi: systemic RNAi mediated gene knockdown in juvenile grasshopper. BMC Biotechnol, 2005, 5:25, 10.1186/1472-6750-5-25, 16202143
Ausubel F M, Brent R, Kingston R E, et al. Short protocols in molecular biology. 3rd ed. Beijing: Science Press, 1998. 414–422
Patel N H. Imaging neuronal subsets and other cell types in whole mount Drosohila embryos and larvae using antibody probes. In: Goldstein L S B, Fyrberg E A, eds. Methods in Cell Biology, Vol. 44. San Diego: Academic Press, 1994. 445–487, 10.1016/S0091-679X(08)60927-9
Chin C T, Chai C H, Sha C Y. Studies on the locust egg I: morphological changes of the embryo during egg incubation, with special reference to the developmental stages of the eggs collected from the field (Acridlldae: Orthoptera). Acta Entomol Sin, 1954, 4:383–398
Isshiki T, Pearson B, Holbrook S, et al. Drosophila neuroblasts sequentially express transcription factors which specify the temporal identity of their neuronal progeny. Cell, 2001, 106:511–521, 1:CAS:528:DC%2BD3MXmsFOiur8%3D, 10.1016/S0092-8674(01)00465-2, 11525736
Jurgens G, Wieschaus E, Nüsslein-Volhard C, et al. Mutations affecting the pattern of the larval cuticle in Drosophila melanogaster. Roux’s Arch Dev Biol, 1984, 193:283–295
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He, Z., Cao, Y., Chen, B. et al. Expression of hunchback during oogenesis and embryogenesis in Locusta migratoria manilensis (Meyen). Sci. China Life Sci. 54, 146–151 (2011). https://doi.org/10.1007/s11427-010-4128-8
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DOI: https://doi.org/10.1007/s11427-010-4128-8