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Characterization of interspecific hybrids and backcross progenies from a cross between Oryza minuta and Oryza sativa

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Abstract

Oryza minuta, a tetraploid wild relative of cultivated rice, is an important source for the genetic improvement. Interspecific hybrids were obtained from the cross of O. sativa L. (IR24) and O. minuta (Acc. No. 101133) with 5.58% crossability, which ranged from 0.11% to 1.62% in the backcross generations. The chromosome numbers of the backcross progenies were 24 to 48. Seven yield-related traits of the parents, hybrid F1, and backcross progenies were evaluated. Simple sequence repeat markers analysis showed that the polymorphism ratio of SSR bands between IR24 and Acc. No. 101133 was 93.2%. The average donor segment number, length, donor genome size, and percentage of donor genome of 92 BC3F1 plants (2n=24) were 24.1, 17.8 cM, 438.4 cM and 26.2%, respectively. They were complex variation and uneven among the chromosomes. These introgression lines could be used to identify the favorable genes of O. minuta and provide a new platform for the genetic improvement of cultivated rice.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan, 430070, China

    SiBin Guo, FaLan Qin, DuanPin Zhang & XingHua Lin

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  1. SiBin Guo
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  2. FaLan Qin
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  3. DuanPin Zhang
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  4. XingHua Lin
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Correspondence to XingHua Lin.

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Supported by the National Natural Science Foundation of China (Grant No. 30370 869)

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Guo, S., Qin, F., Zhang, D. et al. Characterization of interspecific hybrids and backcross progenies from a cross between Oryza minuta and Oryza sativa. SCI CHINA SER C 52, 1148–1155 (2009). https://doi.org/10.1007/s11427-008-0155-0

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