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Chondrogenic differentiation of mouse embryonic stem cells promoted by mature chondrocytes

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Abstract

In order to direct embryonic stem (ES) cells to differentiate into chondrocytes, a chondrogenic environment provided by mature chondrocytes was investigated. Flk-1 positive cells sorted from pre-differentiated mouse ES cells were mixed with adult porcine articular chondrocytes, seeded on biodegradable scaffolds, and then implanted subcutaneously into nude mice. The cell-scaffold complexes formed cartilage tissues after 4 weeks, which was demonstrated by histology and anti-type II collagen antibody staining. Positive staining of mouse Major Histocompatibility Complex class I molecules confirmed that part of the chondrocytes were derived from mouse ES cells. The current study established a new approach for directing ES cell differentiation.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai, 200011, China

    Feng Xie, WenJie Zhang, FanFan Chen, GuangDong Zhou, Lei Cui, Wei Liu & YiLin Cao

  2. National Tissue Engineering Center of China, School of Medicine, Shanghai JiaoTong University, Shanghai, 200011, China

    Feng Xie, WenJie Zhang, FanFan Chen, GuangDong Zhou, Lei Cui, Wei Liu & YiLin Cao

Authors
  1. Feng Xie
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  2. WenJie Zhang
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  3. FanFan Chen
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  4. GuangDong Zhou
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  5. Lei Cui
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  6. Wei Liu
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  7. YiLin Cao
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Corresponding authors

Correspondence to WenJie Zhang or YiLin Cao.

Additional information

Supported by the National Basic Research Program of China (Grant No. 2005CB522705), Shanghai Pujiang Foundation (Grant No. 05PJ14054), National Natural Science Foundation of China (Grant No. 30671051) and Shanghai Shuguang Foundation (Grant No. 06SG22)

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Xie, F., Zhang, W., Chen, F. et al. Chondrogenic differentiation of mouse embryonic stem cells promoted by mature chondrocytes. SCI CHINA SER C 51, 774–778 (2008). https://doi.org/10.1007/s11427-008-0111-z

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  • DOI: https://doi.org/10.1007/s11427-008-0111-z

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