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Genetic diversity of root-nodulating bacteria isolated from pea (Pisum sativum) in subtropical regions of China

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Abstract

Diversity of 42 isolates from effective nodules of Pisum sativum in different geographical regions of China were studied using 16S rRNA gene RFLP patterns, 16S rRNA sequencing, 16S–23S rRNA intergenic spacer (IGS) region RFLP patterns and G-C rich random amplified polymorphic DNA (RAPD). The isolates were distributed in two groups on the basis of their 16S rRNA gene RFLP patterns. The 16S rRNA gene sequences of strains from 16S rRNA gene RFLP patterns group I were very closely related (identities higher than 99.5%) to Rhizobium leguminosarum USDA 2370. Group II consisting of WzP3 and WzP15 was closely related to Rhizobium etli CFN42. The analysis of the 16S-23S IGS RFLP patterns divided the isolates into 18 genotypes and four groups. Group I was clustered with R. leguminosarum USDA2370. Group II consisted of YcP2, YcP3 and CqP7. The strains of group III were distributed abroad. Group IV consisted of WzP3, WzP15 and R. etli CFN42. RAPD divided the isolates into nine clusters in which group IV only consisted of YcP2 and the strains of group V and IX were from Wenzhou and Xiantao, respectively. This assay demonstrated the geographical effect on genetic diversity of pea rhizobia.

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Authors and Affiliations

  1. State Key Laboratory of Agro-Microbiology, Huazhong Agricultural University, Wuhan, 430070, China

    ChengYun Yang, YouGuo Li & JunChu Zhou

  2. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China

    JiangKe Yang

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  1. ChengYun Yang
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  4. JunChu Zhou
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Correspondence to JunChu Zhou.

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Supported by the Chinese Microbe Resource Project (Grant No. 2005DKA21208-6), Chinese High-tech Developing Program (Grant No. 2007AA05Z417), and the Opening Foundation of State Key Laboratory of Agricultural Microbiology, HAU, China

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Yang, C., Yang, J., Li, Y. et al. Genetic diversity of root-nodulating bacteria isolated from pea (Pisum sativum) in subtropical regions of China. SCI CHINA SER C 51, 854–862 (2008). https://doi.org/10.1007/s11427-008-0104-y

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  • DOI: https://doi.org/10.1007/s11427-008-0104-y

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