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Molecular phylogeny of Pneumocystis based on 5.8S rRNA gene and the internal transcribed spacers of rRNA gene sequences

Science in China Series C: Life Sciences volume 51, pages 445–452 (2008)Cite this article

Abstract

To clarify the phylogenetic relationships and species status of Pneumocystis, the 5.8S rRNA gene and the internal transcribed spacers (ITS, 1 and 2) of Pneumocystis rRNA derived from rat, gerbil and human were amplified, cloned and sequenced. The genetic distance matrix of six Pneumocystis species compared with other fungi like Taphrina and Saccharomyces indicated that the Pneumocystis genus contained multiple species including Pneumocystis from gerbil. The phylogenetic tree also showed that Pneumocystis from human and monkey formed one group and four rodent Pneumocystis formed another group. Among the four members, Pneumocystis wakefieldiae was most closely related to Pneumocystis murina and Pneumocystis carinii, and was least related to gerbil Pneumocystis.

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Affiliations

  1. Department of Pathogenic Biology, Capital Medical University, Beijing, 100069, China

    ZiHui Li, XianMin Feng, SiQi Lu, Fan Zhang, FengYun Wang & Song Huang

  2. Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, 101100, China

    ZiHui Li

Authors
  1. ZiHui Li
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  2. XianMin Feng
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  3. SiQi Lu
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  4. Fan Zhang
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  5. FengYun Wang
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  6. Song Huang
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Corresponding author

Correspondence to SiQi Lu.

Additional information

Supported by the Natural Science Foundation of Beijing, China (Grant No. 7052009) and the National Natural Science Foundation of China (Grant No. 30470243)

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Li, Z., Feng, X., Lu, S. et al. Molecular phylogeny of Pneumocystis based on 5.8S rRNA gene and the internal transcribed spacers of rRNA gene sequences. SCI CHINA SER C 51, 445–452 (2008). https://doi.org/10.1007/s11427-008-0057-1

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  • DOI: https://doi.org/10.1007/s11427-008-0057-1

Keywords

  • Pneumocystis
  • phylogeny
  • 5.8S rRNA gene
  • internal transcribed spacers