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Molecular phylogeny of Pneumocystis based on 5.8S rRNA gene and the internal transcribed spacers of rRNA gene sequences

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Abstract

To clarify the phylogenetic relationships and species status of Pneumocystis, the 5.8S rRNA gene and the internal transcribed spacers (ITS, 1 and 2) of Pneumocystis rRNA derived from rat, gerbil and human were amplified, cloned and sequenced. The genetic distance matrix of six Pneumocystis species compared with other fungi like Taphrina and Saccharomyces indicated that the Pneumocystis genus contained multiple species including Pneumocystis from gerbil. The phylogenetic tree also showed that Pneumocystis from human and monkey formed one group and four rodent Pneumocystis formed another group. Among the four members, Pneumocystis wakefieldiae was most closely related to Pneumocystis murina and Pneumocystis carinii, and was least related to gerbil Pneumocystis.

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References

  1. Chagas C. Nova tripanozomiaze humana: Estudos sobre a morfolojia eo ciclo evolutivo do schizotrypanum cruzi n. gen., n. sp., agente etiolojico de nova entidade morbida do homen. Mem Inst Oswaldo Cruz, 1909, 1: 159–218

    Article  Google Scholar 

  2. Edman J C, Kovacs J A, Masur H, et al. Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the fungi. Nature, 1988, 334(6182): 519–522 2970013, 10.1038/334519a0, 1:CAS:528:DyaL1cXlt1Gltb8%3D

    Article  CAS  Google Scholar 

  3. Denis C M, Mazars E, Guyot K, et al. Genetic divergence at the SODA locus of six different formae speciales of Pneumocystis carinii. Med Mycol, 2000, 38(4): 289–300 10975697, 10.1080/714030952, 1:CAS:528:DC%2BD3cXmslSnsro%3D

    Article  CAS  Google Scholar 

  4. Keely S, Pai H J, Baughman R, et al. Pneumocystis species inferred from analysis of multiple genes. J Eukaryot Microbiol, 1994, 41(5): 94

    Google Scholar 

  5. Guillot J, Demanche C, Hugot JP, et al. Parallel phylogenies of Pneumocystis species and their mammalian hosts. J Eukaryot Microbiol, 2001(Suppl):113–115

  6. Demanche C, Berthelemy M, Petit T, et al. Phylogeny of Pneumocystis carinii from 18 primate species confirms host specificity and suggests coevolution. J Clin Microbiol, 2001, 39(6): 2126–2133 11376046, 10.1128/JCM.39.6.2126-2133.2001, 1:CAS:528:DC%2BD3MXkslCks7w%3D

    Article  CAS  Google Scholar 

  7. Keely S P, Fischer J M, Cushion M T, et al. Phylogenetic identification of Pneumocystis murina sp. nov., a new species in laboratory mice. Microbiology, 2004, 150(Pt): 1153–1165 15133075, 10.1099/mic.0.26921-0, 1:CAS:528:DC%2BD2cXktlOlsr4%3D

    Article  CAS  Google Scholar 

  8. Totet A, Pautard JC, Raccurt C, et al. Genotypes at the internal transcribed spacers of the nuclear rRNA operon of Pneumocystis jiroveci in nonimmunosuppressed infants without severe pneumonia. J Clin Microbiol 2003, 41(3): 1173–1180 12624048, 10.1128/JCM.41.3.1173-1180.2003, 1:CAS:528:DC%2BD3sXisF2msL0%3D

    Article  CAS  Google Scholar 

  9. Hosoya N, Takahashi T, Wada M, et al. Genotyping of Pneumocystis carinii f. sp. hominis isolates in Japan based on nucleotide sequence variations in internal transcribed spacer regions of rRNA genes. Microbiol Immunol, 2000, 44(7): 591–596 10981832, 1:CAS:528:DC%2BD3cXmsVyhs74%3D

    Article  CAS  Google Scholar 

  10. Lee C H, Helweg-Larsen J, Tang X, et al. Update on Pneumocystis carinii f. sp. hominis typing based on nucleotide sequence variations in internal transcribed spacer regions of rRNA genes. J Clin Microbiol, 1998, 36(3): 734–741 9508304, 1:CAS:528:DyaK1cXhsVGitbo%3D

    CAS  Google Scholar 

  11. Nimri L F, Moura I N, Huang L, et al. Genetic diversity of Pneumocystis carinii f. sp. hominis based on variations in nucleotide sequences of internal transcribed spacers of rRNA genes. J Clin Microbiol, 2002, 40(4): 1146–1151 11923323, 10.1128/JCM.40.4.1146-1151.2002, 1:CAS:528:DC%2BD38XjtlCnur0%3D

    Article  CAS  Google Scholar 

  12. Sambrook J, Russell D W. Molecular cloning: A Laboratory Manual. 3rd ed. New York: Cold Spring Harbor Laboratory, 2001. 463–470

    Google Scholar 

  13. Hua L, Qin S, Wang A, et al. The diagnostic value of polymerase chain reaction for the detection of Pneumocystis carinii DNA from induced sputum samples. Chin J Intern Med, 2002, 41(9): 610–612 1:CAS:528:DC%2BD3sXpsFWqtb0%3D

    CAS  Google Scholar 

  14. Li J, Lu S, Zhang Y, et al. Molecular phylogeny of Giardia lamblia based on triose phosphates isomerase (tim) gene sequence. Chin J Parasitol Parasit Dis, 2000, 18(3): 141–145 1:STN:280:DC%2BD3s%2Flsl2rsA%3D%3D

    CAS  Google Scholar 

  15. Nei M, Kumar S. Molecular evolution and phylogeny. Beijing: Higher Education Press 2002. 151–153

    Google Scholar 

  16. Robberts F J, Liebowitz L D, Chalkley L J. Genotyping and coalescent phylogenetic analysis of Pneumocystis jiroveci from South Africa. J Clin Microbiol, 2004, 42(4): 1505–1510 15070996, 10.1128/JCM.42.4.1505-1510.2004, 1:CAS:528:DC%2BD2cXjvFylsbY%3D

    Article  CAS  Google Scholar 

  17. Taylor J W, Bowman B H. Pneumocystis carinii and the ustomycetous red yeast fungi. Mol Microbiol, 1993, 8(2): 425–427 8316090, 10.1111/j.1365-2958.1993.tb01585.x, 1:CAS:528:DyaK3sXkt1Cntrc%3D

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathogenic Biology, Capital Medical University, Beijing, 100069, China

    ZiHui Li, XianMin Feng, SiQi Lu, Fan Zhang, FengYun Wang & Song Huang

  2. Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, 101100, China

    ZiHui Li

Authors
  1. ZiHui Li
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  2. XianMin Feng
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  3. SiQi Lu
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  4. Fan Zhang
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  5. FengYun Wang
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  6. Song Huang
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Corresponding author

Correspondence to SiQi Lu.

Additional information

Supported by the Natural Science Foundation of Beijing, China (Grant No. 7052009) and the National Natural Science Foundation of China (Grant No. 30470243)

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Li, Z., Feng, X., Lu, S. et al. Molecular phylogeny of Pneumocystis based on 5.8S rRNA gene and the internal transcribed spacers of rRNA gene sequences. SCI CHINA SER C 51, 445–452 (2008). https://doi.org/10.1007/s11427-008-0057-1

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  • DOI: https://doi.org/10.1007/s11427-008-0057-1

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