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Recruitment of stem cells by hepatocyte growth factor via intracoronary gene transfection in the postinfarction heart failure

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Abstract

We aim to study the amelioration effect of adenovirus5-mediated human hepatocyte growth factor gene transfer on postinfarction heart failure in swine model. Twelve Suzhong young swine were randomly divided into 2 groups of 6 pigs each: Ad5-HGF group and mock-vector Ad5 group. Four weeks after ligation of the left anterior descending coronary artery, Ad5-HGF was intracoronarily transferred into the myocardium. Simultaneously, gate cardiac perfusion imaging was performed to evaluate the heart function. Three weeks later, gate cardiac perfusion imaging was performed again, then the hearts were removed and sectioned for immunohistochemical examination to illustrate the effects of Ad5-HGF on infarcted myocardium. The expression of HGF was examined by ELISA. The results were: (1) compared with the mock-vector Ad5 group, high expression of human HGF was observed in the myocardium of Ad5-HGF group; (2) in the Ad5-HGF group, the number of CD117+ cells co-expressing c-Met per mm2 was significantly larger; (3) the improvement in LVEF was greater in the Ad5-HGF group than in the mock-vector Ad5 group. We concluded that: (1) high expression of human HGF was observed in the myocardium through intracoronary gene transfection; (2) HGF can improve the mobilization of CD117+/c-Met+ stem cells into ischemic myocardium. The amelioration effect of HGF on postinfarction heart failure could not be limited to stimulating angiogenesis, anti-apoptosis, anti-fibrosis, but was also involved in the recruitment of stem cells into myocardium.

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Authors and Affiliations

  1. Department of Cardiovascular Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China

    Yang ZhiJian, Wang Wei, Ma DongChao, Wang LianSheng, Zhang YuQing, Xu ShunLin, Chen Bo, Cao KeJiang & Ma WenZhu

  2. Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing, 100850, China

    Zhang YouRong

  3. Department of Experimental anatomy, Nanjing Medical University, Nanjing, 210029, China

    Miao DengShun

Authors
  1. Yang ZhiJian
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  2. Wang Wei
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  3. Ma DongChao
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  4. Zhang YouRong
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  5. Wang LianSheng
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  6. Zhang YuQing
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  7. Xu ShunLin
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  8. Chen Bo
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  9. Miao DengShun
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  10. Cao KeJiang
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  11. Ma WenZhu
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Correspondence to Yang ZhiJian.

Additional information

Supported by the Medical Key Member of “Medicine Renaissance Projects of Jiangsu Province” to Yang Z J and the Chinese National High Technology Development Program (863 Program) (Grant No. 2004CB518801)

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Yang, Z., Wang, W., Ma, D. et al. Recruitment of stem cells by hepatocyte growth factor via intracoronary gene transfection in the postinfarction heart failure. SCI CHINA SER C 50, 748–752 (2007). https://doi.org/10.1007/s11427-007-0102-5

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  • DOI: https://doi.org/10.1007/s11427-007-0102-5

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