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Discovery and identification of Serum Amyloid A protein elevated in lung cancer serum

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Abstract

Two hundred and eighteen serum samples from 175 lung cancer patients and 43 healthy individuals were analyzed by using Surface Enhaced Laser Desorption/Ionization Time of Flight Mass Spectrome-try (SELDI-TOF-MS). The data analyzed by both Biomarker Wizard™ and Biomarker Patterns™ software showed that a protein peak with the molecular weight of 11.6 kDa significantly increased in lung cancer. Meanwhile, the level of this biomarker was progressively increased with the clinical stages of lung cancer. The candidate biomarker was then obtained from tricine one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis by matching the molecular weight with peaks on WCX2 chips and was identified as Serum Amyloid A protein (SAA) by MALDI/MS-MS and database searching. It was further validated in the same serum samples by immunoprecipitation with commercial SAA antibody. To confirm the SAA differential expression in lung cancer patients, the same set of serum samples was measured by ELISA assay. The result showed that at the cutoff point 0.446 (OD value) on the Receiver Operating Characteristic (ROC) curve, SAA could better discriminate lung cancer from healthy individuals with sensitivity of 84.1% and specificity of 80%. These findings demonstrated that SAA could be characterized as a biomarker related to pathological stages of lung cancer.

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Abbreviations

SAA:

Serum Amyloid A protein

ELISA:

enzyme linked immunosor-bent assay

ROC:

receiver operating characteristic

SELDI-TOF-MS:

Surface-Enhanced Laser Desorption/Ionization-Time of Flight-Mass Spectrometry

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Authors and Affiliations

  1. Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Beijing Normal University, Beijing, 100875, China

    Dai SongWei, Wang XiaoMin, Liu LiYun, Huang LingYun, Xiao XueYuan & He DaCheng

  2. Universities’ Confederated Institute of Proteomics, Beijing, 100875, China

    Dai SongWei, Wang XiaoMin, Liu LiYun, Huang LingYun, Xiao XueYuan & He DaCheng

  3. Department of Thoracic Surgery, General Hospital of Beijing Unit, PLA, Beijing, 100500, China

    Liu JiFu & Wu ShanShan

Authors
  1. Dai SongWei
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  2. Wang XiaoMin
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  3. Liu LiYun
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  4. Liu JiFu
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  5. Wu ShanShan
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  6. Huang LingYun
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  7. Xiao XueYuan
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  8. He DaCheng
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Corresponding authors

Correspondence to Xiao XueYuan or He DaCheng.

Additional information

Supported in part by the Major State Basic Research Program of China (Grant No. 2006CB910100), National Natural Science Foundation of China (Grant No. 30370712), Natural Science Foundation of Beijing (Grant No. 7051002), and Science Technology Committee of Beijing (Grant No.Y0204002040111

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Dai, S., Wang, X., Liu, L. et al. Discovery and identification of Serum Amyloid A protein elevated in lung cancer serum. SCI CHINA SER C 50, 305–311 (2007). https://doi.org/10.1007/s11427-007-0053-x

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  • DOI: https://doi.org/10.1007/s11427-007-0053-x

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