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The guanidine hydrochloride-induced denaturation of CP43 and CP47 studied by terahertz time-domain spectroscopy

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Abstract

Terahertz time-domain spectroscopy (THz-TDS) is a new technique in studying the conformational state of a molecule in recent years. In this work, we reported the first use of THz-TDS to examine the denaturation of two photosynthesis membrane proteins: CP43 and CP47. THz-TDS was proven to be useful in discriminating the different conformational states of given proteins with similar structure and in monitoring the denaturation process of proteins. Upon treatment with guanidine hydrochloride (GuHCl), a 1.8 THz peak appeared for CP47 and free chlorophyll a (Chl a). This peak was deemed to originate from the interaction between Chl a and GuHCl molecules. The Chl a molecules in CP47 interacted with GuHCl more easily than those in CP43.

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Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Qu YuanGang, Qin XiaoChun, Li LiangBi & Kuang TingYun

  2. Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing, 100080, China

    Chen Hua & Wang Li

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Qu YuanGang

Authors
  1. Qu YuanGang
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  2. Chen Hua
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  3. Qin XiaoChun
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  4. Wang Li
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  5. Li LiangBi
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  6. Kuang TingYun
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Corresponding authors

Correspondence to Li LiangBi or Kuang TingYun.

Additional information

Supported by the National Natural Science Foundation of China (Grant No. 39890390)

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Qu, Y., Chen, H., Qin, X. et al. The guanidine hydrochloride-induced denaturation of CP43 and CP47 studied by terahertz time-domain spectroscopy. SCI CHINA SER C 50, 350–355 (2007). https://doi.org/10.1007/s11427-007-0048-7

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  • DOI: https://doi.org/10.1007/s11427-007-0048-7

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