Skip to main content
SpringerLink
Log in

Comparison between gene expression of conidia and germinating phase in Trichophyton rubrum

  • Published:
Science in China Series C: Life Sciences Aims and scope Submit manuscript

Abstract

Trichophyton rubrum is a dominating superficial dermatophyte, whose conidial germination is correlated to pathopoiesis and a highly important developmental process. To investigate the changes of physiology, biochemistry and cytology during the germination, we selected 3364 function identified ESTs from T. rubrum cDNA library to construct cDNA microarrays, and compared the gene expression levels of conidia and germinating phase. Data analysis indicated that 335 genes were up-regulated during the germination, which mainly encoded translated, modified proteins and structural proteins. The constituents of cell wall and cell membrane were synthetized abundantly, suggesting that they are the foundation of cell morphogenesis. The ingredients of the two-component signal transduction system were up-regulated, presuming that they were important for the conidial germination. Genes of various metabolic pathways were expressed prosperously, especially the genes that participated in glycolysis and oxidative phosphorylation were up-regulated on the whole, demonstrating that in the environment with sufficient oxygen and glucose, conidia obtained energy through aerobic respiration. This paper provides important clues which are helpful to understanding the changes in gene expression, signal conduction and metabolism characteristics during T. rubrum conidial germination, and possess significant meaning to the study of other superficial dermatophytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wen Y M, Lu D Y, He L F. Modern Medical Microbiology (in Chinese). Shanghai: Shanghai Medical University Press, 2000. 677–728

    Google Scholar 

  2. Wang L L, Ma L, Leng W C, et al. Analysis of part of the Trichophyton rubrum ESTs. Sci China Ser C-Life Sci, 2004, 47: 389–395

    Article  Google Scholar 

  3. Borkovich K A, Alex L A, Yarden O, et al. Lessons from the genome sequence of Neurospora crassa: Tracing the path from genomic blueprint to multicellular organism. Microbiol Mol Biol R, 2004, 68(1): 1–108

    Article  CAS  Google Scholar 

  4. Galagan J E, Calvo S E, Cuomo C, et al. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature, 2005, 438: 1105–1115

    Article  PubMed  CAS  Google Scholar 

  5. Chambergo F S, Bonaccorsi E D, Ferreira A J S, et al. Elucidation of the metabolic fate of glucose in the filamentous fungus Trichoderma reesei using expressed sequence tag (EST) analysis and cDNA microarrays. J Biol Chem, 2002, 277(16): 13983–13988

    Article  PubMed  CAS  Google Scholar 

  6. Schena M, Shalon D, Davis R W, et al. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science, 1995, 270(5235): 467–470

    Article  PubMed  CAS  Google Scholar 

  7. Kanehisa M, Goto S, Kawashima S, et al. The KEGG resource for deciphering the genome. Nucleic Acids Res, 2004, 32 (Database issue): 277–280

    Article  Google Scholar 

  8. Peng J P, Zhang X B, Yang J, et al. The use of comparative genomic hybridization to characterize genome dynamics and diversity among the serotypes of Shigella. BMC Genomics, 2006, 7: 218

    Article  PubMed  Google Scholar 

  9. He X P, Zhang B R. Research advancement of ergosterol in microbes. Microbiol Bull (in Chinese), 1998, 25(3): 166

    Google Scholar 

  10. Kasuga T, Townsend J P, Tian C G, et al. Long-oligomer microarray profiling in Neurospora crassa reveals the transcriptional program underlying biochemical and physiological events of conidial germination. Nucleic Acids Res, 2005, 33(20): 6469–6485

    Article  PubMed  CAS  Google Scholar 

  11. Galagan J E, Calvo S E, Borkovich K A, et al. The genome sequence of the filamentous fungus Neurospora crassa. Nature, 2003, 422: 859–868

    Article  PubMed  CAS  Google Scholar 

  12. Lengeler K B, Davidson R C, Souza C D, et al. Signal transduction cascades regulating fungal development and virulence. Microbiol Mol Biol R, 2000, 64(4): 746–785

    Article  CAS  Google Scholar 

  13. Calera J A, Zhao X J, Calderone R. Defective hyphal development and avirulence caused by a deletion of the SSK1 response regulator gene in Candida albicans. Infect Immun, 2000, 68(2): 518–525

    Article  PubMed  CAS  Google Scholar 

  14. Brinkman F S L, Macfarlane E L A, Warrener P, et al. Evolutionary relationships among virulence-associated histidine kinases. Infect Immun, 2001, 69(8): 5207–5211

    Article  PubMed  CAS  Google Scholar 

  15. Koretke K K, Lupas A N, Warren P V, et al. Evolution of two-component signal transduction. Mol Biol Evol, 2000, 17(12): 1956–1970

    PubMed  CAS  Google Scholar 

  16. Catlett N L, Yoder O C, Turgeon B G. Whole-genome analysis of two-component signal transduction genes in fungal pathogens. Eukaryotic Cell, 2003, 2(6): 1151–1161

    Article  PubMed  CAS  Google Scholar 

  17. Alex L A, Borkovich K A, Simon M I. Hyphal development in Neurospora crassa: Involvement of a two-component histidine kinase. Proc Natl Acad Sci USA, 1996, 93: 3416–3421

    Article  PubMed  CAS  Google Scholar 

  18. Hohmann S. Osmotic stress signaling and osmoadaptation in yeasts. Microbiol Mol Biol R, 2002, 66(2): 300–372

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Life Sciences, Wuhan University, Wuhan, 430072, China

    Yang Li, Qi YiPeng & Jin Qi

  2. State Key Laboratory for Molecular Virology and Genetic Engineering, Chinese Center for Disease Control and Prevention, Beijing, 100176, China

    Yang Li, Wang LingLing, Peng JunPing, Yu Lu, Liu Tao, Leng WenChuan, Yang Jian, Chen LiHong, Zhang WenLiang, Zhang Qian & Jin Qi

  3. Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, 100730, China

    Jin Qi

Authors
  1. Yang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wang LingLing
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peng JunPing
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Liu Tao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Leng WenChuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yang Jian
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Chen LiHong
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Zhang WenLiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Zhang Qian
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Qi YiPeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Jin Qi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin Qi.

Additional information

Supported by the National High Technology Research and Development Program of China (Grant No. 2001AA223021) and National Key Technologies R&D Programme (Grant No. 2002BA711A14)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, L., Wang, L., Peng, J. et al. Comparison between gene expression of conidia and germinating phase in Trichophyton rubrum . SCI CHINA SER C 50, 377–384 (2007). https://doi.org/10.1007/s11427-007-0046-9

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11427-007-0046-9

Keywords

Search

Navigation