Abstract
In order to understand the allograft rejection in orthotopic liver transplantation (OLT), an allograft rejection rat model was established and studied by proteomic approach. The protein expression profiles of liver tissues were acquired by fluorescence two-dimensional difference gel electrophoresis (2D DIGE) that incorporated a pooled internal standard and reverse fluorescent labeling method. The expression levels of 27 protein spots showed significant changes in acute rejection rats. Among these spots, 19 were identified with peptide mass fingerprinting using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) after tryptic in-gel digestion. The results of the present paper could be helpful for our better understanding of allograft rejection in organ transplantation.
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Abbreviations
- Cy2:
-
3-[(4-carboxymethyl)-phenylmethyl]-3′-ethyloxacarbocyanine halide N-hydroxysuccinimidyl ester
- Cy3:
-
1-(5-carboxypentyl)-1′-propylindocarbocyanine halide N-hydroxysuccinimidyl ester
- Cy5:
-
1-(5-carboxypentyl)-1′-methylindodicarbo-cyanine halide N-hydroxysuccinimidyl ester
- OLT:
-
orthotopic liver transplantation
- ACN:
-
acetonitrile
- TFA:
-
trifluroracetic acid
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Supported by the Key Basic Research and Development Program of China (Grant No. 2003CB515506), and in part by the Chinese Human Liver Proteome Project (CNHLPP) (Grant No. 2004BA711A18)
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Zhang, C., Zhu, F., Wei, J. et al. A proteomic analysis of allograft rejection in rats after liver transplantation. SCI CHINA SER C 50, 312–319 (2007). https://doi.org/10.1007/s11427-007-0038-9
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DOI: https://doi.org/10.1007/s11427-007-0038-9